Coating glass with Heparin?

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COW Algorithm R Package for GC-FID Pre-processing?

Hi! Are there any R packages or Python modules that are able to implement the COW algorithm in chromatogram alignment? Thanks!

13 July 2023 1,926 0 View

How do you deal with negative values in predictor and independent variable in regression?

I am trying to run an OLS regression, with two continuous variables, both with negative and positive values. How should one deal with this? How do you plot a regression line in this case? Is it...

29 December 2022 5,945 4 View

I am running RegCM4.7.1 with ERA5, Terrain and SST was successfully but when I run ICBC, it returns:No timesteps for SST data in SST file. Why?

This is icbc part of the RegCM version 4 SVN Revision: 4.7.1-1027-g0c9c8 compiled at: data : Oct 8 2021 time: 04:29:07 : this run start at : 2022-12-30 04:39:43+0000...

29 December 2022 930 18 View

Recent issues with Sylgard 184 for microfluidics?

Hi, Has anyone doing microfluidics work for cell culture started having problems recently with getting their Sylgard 184 PDMS to reliably and permanently bond to the glass substrate? We have tried...

10 July 2022 7,822 1 View

Looking for cell viability stains for flow sorting, any ideas?

Hi, we are currently doing a CRISPRi screen in THP-1 cells. Our first transduction is with TA3 lentiviruses. These carry Blasticidin resistance. The only way to collect these cells, and to...

31 May 2022 5,325 2 View

How do you ensure single integration of sgRNAs when doing a CRISPR screen?

I know I am looking for an MOI of 0.3, but I am trying to figure out how one can ensure this.

05 April 2022 1,466 2 View

What cell lines can I use?

I am looking for a macrophage human cell line. Preferably one that is already adherent vs a cell line that requires differentiation.

30 March 2022 757 5 View

Coding an Emotional Stop Signal Task in E-Prime 3.0 with variable stop signal delay?

Hello All! I am working on implementing an Emotional Stop Signal Task in E-Prime 3.0. Validation of an emotional stop-signal task to probe individ... Originally my plan was to modify the...

20 March 2022 1,658 1 View

Has anyone found the use of COVID-19 disposable masks increased microfiber contamination in microplastic lab samples?

I am an undergraduate student learning the ropes of microplastic sample processing and have found that my blanks continue to have above average counts of fiber contamination. We have found that...

18 October 2021 2,714 2 View

Know any gas chromatogram alignment software?

Hello! I have a lot of chromatograms with hundreds of peaks that need to be aligned. Can you recommend software (hopefully, opensource) that I can use to align peaks? Thanks!

10 September 2021 2,240 5 View

Adhesion strength of coating?

How can I determine a good adhesion strength range for coatings on polymer surfaces, such as DLC on polymer substrates? Is there a specific threshold for adhesion strength (from T-peel tests)...

10 August 2024 942 3 View

How do I conformally do PR spin coating on trench structure?

I did PR spin coating on trench structure. I used AZ P4620 PR and the thickness or PR is around 11um. The substrate is Si. And my trench structure depth is 141um(negative way). Even though I...

08 August 2024 2,298 0 View

Fabrication of narrow glass capillary (inner diameter 200~500 nm)?

I need a very narrow glass capillary for my research. How can I make a glass capillary with an inner diameter of around 200 to 500 nanometers?

08 August 2024 8,915 0 View

Why do i have a weak plasma and no coating during rf sputtering?

I have been getting a low plasma and no coating while doing rf sputtering of copper doped ZnS using a power of 140 watts and at pressure of 6.5x10-3 Mb.

07 August 2024 4,526 4 View

What is the reason I get 400nm nanoparticles using an extruder with 200 nm membrane for cell membrane coating?

I am trying to coat my micelles and PLGA based nanoparticles with cell membrane using an Avanti mini extruder according to literature . I use 200nm membrane but my particles end up to be 400nm And...

30 July 2024 3,294 3 View

What is the main used curing agent for epoxy based coating?

I am trying to coating metal oxide into steel substrate, for better sticking planning to utilse epoxy based, Could you please suggest suitable curing agent for the coating purpose

25 July 2024 438 3 View

How do you get pure primary macrophage cell culture?

I have been harvesting monocyte-derived macrophages from buffalo and cattle fresh whole blood using the same protocol for years and getting good macrophage cultures. Below is the protocol that I...

23 July 2024 4,074 2 View

Does the potentiostat have to be in a fume hood when polymerising the PANI?

I will polymerise Aniline on ITO glass WE, using 1M of H2SO4 and a potentiostat. However, I am not sure if I have to do the electrochemical polymerisation inside a hood. Is it necessary? Is the...

10 July 2024 2,937 3 View

Issues in coating of cathode material on Al foil, why we are not getting proper coating and results when the cathode was coated on Al-foil..?

We are trying to coat some cathode materials on Al-foil ( ex - LiFePO4 , S@CNT, etc). When we coated the Al foil with the cathode (ex - S@CNT) material , the cycling result was annoying. But the...

09 July 2024 5,123 4 View

Why NCM cathode slurry has coating problem?

I tried to make slurry for NCM cathode but there was problem in coating process. The detail of my experiment is following; The ratio between active material(NCM811), conductive additives(Super-P)...

08 July 2024 7,327 4 View

Amin Shavandi

Please check for the Carmeda heparin binding approach that employs a single covalent bond between the reducing end of the heparin molecule and the material substrate such as glass and polymer.

Abigail Mensah Forson

Thank you Amin

Andrey A Tyuftin

Plasma treatment should work as well of the glass surface, it will make it more hydrophilic.