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Is it possible to activate these models with the presence of e-learning at the present time or are they somewhat difficult to implement?

29 December 2020 1,076 12 View

Is there anyone interested in a colaborative work to find a vaccine for COVID-19?

I have proposed a new multiscale model that it is just recently published. The new multiscale model suggests a new method to solve multiscale problems. The important thing is this model predicts...

26 March 2020 1,748 7 View

Hi all.Iam ask about the biochemistry journal for publishing my article in short time.but the journal must be sign in scoups?

My article asociated with aschemic diseases

22 July 2018 8,659 2 View

Please ,which are types of bacteria is resistant to antibiotic (ATI) and can be inactivation by cold plasma.?

There are many bacteria that resist antibiotics and cold plasma is a promising way to inactivate these types of bacteria

03 May 2018 7,359 4 View

Is it valid to use total Confusion Matrix for k-folds cross-validation and m-times of repeats?

I would like to use the confusion matrix as a measure of classification accuracy. So, I wonder whether is it reasonable to find the accumulative confusion matrix for k-folds and m-times of repeats...

30 December 2017 5,127 1 View

How to estimate the effect of UV-C type on humans?

Electromagnetic radiation has general ?effects on humans, including ultraviolet radiation type UV-C,such as skin diseases and dark eye

03 December 2017 4,211 3 View

Need some atricals or software?

I need some codes in matlab to generation observations of bivariate pareto.. If any one have atricals or software send me My thanks to all with best regards

01 November 2017 1,566 1 View

The main differneces between wit.ai, api.ai, and LUIS ?

Anyone performed or knows some resources describing the main differneces between wit.ai, api.ai, and LUIS ? Any papers, datasets, or articles would be appreciated. Thank you

21 March 2017 159 2 View

Where can I find food logs, daily dietary activity, or diet tracking dataset openly available ?

I need to run offline experimentation about user's daily dietary activity and find a way to classify them based on their adherence.

17 November 2016 1,936 8 View

How can we recognize the glassy phases in aluminum through XRD?

07 May 2016 5,010 5 View

Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

03 March 2021 401 4 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View

Difficulty in cloning of 1kb gene into vector?

I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...

25 February 2021 3,221 7 View

Why are Sanger Sequencing reads interrupted in some edited clones?

I have a Crispr/Cas9 edited bulk population and I can briefly check the indel ratios in the bulk population using Sanger sequencing. After cloning single cells, I wanted to check the indel types...

18 February 2021 8,198 6 View

Hi, can anybody help me with the suggestions how can I amplify a stress induced gene of 1107 bp?

I am trying to amplify the TGA1 (Transcription factor 1 ) of Arabidopsis ,which is 1107 bp. I have treated the plants with Salycylic solution over night and extracted the RNA and make cDNA. I...

17 February 2021 7,802 2 View

Alternatives to traditional cloning of insert into backbone (dsDNA)?

Hi, I am stuck with a cloning experiment: The insert & backbone(bb) share one compatible end at 3' end(NotI) and one incompatible end at 5' end (XbaI for insert; HindIII for backbone). So i...

16 February 2021 1,664 3 View

Why do I have trouble with cloning duplicate gene in the same plasmid?

Hello! I am trying to generate a construct in this format: seqA - seqB - linker - seqC - seqB into pSF-CMV-Ub-Puro-Ascl plasmid 0.5kb 2kb 66 0.25kb 2kb I have tried many...

15 February 2021 7,118 3 View

Would using an asRNA for repressing transcription of a high expression Listeria monocytogenes non-coding RNA harbored in a plasmid be a viable option?

Hello all, I am interested in deleting a highly expressed non-coding RNA located on a Listeria monocytogenes plasmid. Currently, there are not any optimized gene deletion methods for Listeria...

09 February 2021 4,994 1 View

Quality of antibodies from Creative Biolabs?

It seems Creative Biolabs has everything and so many versions of antibodies not owned or sold by any other company. How reliable are their products? I am looking for a clone where potentially...

08 February 2021 2,167 3 View