We have a doxycycline-inducible system for the over expression of a gene of interest downstream of TRE3G promotor. While designing the empty vector control, I realised that it means deleting the gene of interest and leaving it empty downstream of the TRE3G promoter. To me this strategy is fine if I introduce a stop codon.

However, are there any potential problems? Is it better to insert a catalytically inactive mutant downstream of TRE3G promoter?