Echo Xiao

3 Questions 1 Answers 0 Followers

Questions related from Echo Xiao

Does mutation in long terminal repeat (LTR) regions of lentiviral plasmid affect gene delivery?

Hi lentivirus experts, I have lentivirus plasmids that carry mutations in the 3' and 5' LTR region. These regions were not exactly designed for easy cloning. Therefore, I wonder if it is possible...

15 April 2024 3,031 2 View

How to optimize tumor digestion protocol to avoid clumps?

Hi all, I work with the MC38 colorectal cancer model and the goal is to dissociate tumor infiltrating immune cells with minimal loss. There is also massive tumor cell death if a therapy is...

24 October 2023 3,710 1 View

Cloning: leaving it empty downstream of TRE3G promotor?

We have a doxycycline-inducible system for the over expression of a gene of interest downstream of TRE3G promotor. While designing the empty vector control, I realised that it means deleting the...

13 June 2022 342 0 View