Dear researchers,
I have a question about cloning and gene expression of chitinase enzymes from shrimp.
Is the expression of shrimp chitinase gene in what kind of bacterial strain?
Thank you for your answers
1- bacterial fermentation with shrimp pond waste water as media source for chitinase production by bacteria industrial strains .
I have not heard anything about shrimp chitinase ,but you can design your study according to :
1- bacterial fermentation with shrimp pond waste water as media source for chitinase production by bacteria industerial strains .
2- exo chitinase (production & properties) comparison between fungi and bacteria
>>>> for recombinant chitinase production , you can used E.coli BL21(DE3) or E.coli origami(DE3) ...
Article Optimized expression conditions for enhancing production of ...
" Characterization of a cold-adapted and salt-tolerant exo-chitinase (ChiC) from Pseudoalteromonas sp. DL-6 "
"Trichoderma asperellum exo chitinase , chitinase B (ChiB) from Serratia marcescens "
"Production of Chitinase and its Optimization from a Novel Isolate Serratia marcescens XJ-01"
"Bacillus licheniformis was found to produce maximum chitinase."
Article Cloning, expression, purification and characterisation of a ...
Article Production of a Thermostable and Alkaline Chitinase by Bacil...
https://pdfs.semanticscholar.org/d26d/64ddac3c781d50b905afcc8d30688d2e4f02.pdf
https://pdfs.semanticscholar.org/063d/db589e2b3b3e5e7c7c3eda51b5cdcd60d3db.pdf
http://jifro.areo.ir/article_114253_a0281d43d5e651cab5910752d7581dfc.pdf
good luck
hi
I have not heard anything about this(shrimp chitinase), but you can design your research topic as high:
I think E coli can be used for tranformation of plasmid containg your gene of interest . It is better to say which plasmid and promotor is the best for the expression.
Pichia pastoris can also be used as a host strain as well.
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