Clean room trace analysis lab cleaning solutions?

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How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Can I project all raw motor imagery EEG data onto two spatial filters developed from training data?

Greetings RG community, I have been working on a pipeline for the classification of EEG motor imagery signals. This is currently being done on the Giga Science MI dataset with 52 subjects, using...

28 January 2021 6,947 2 View

How to calculate error for a Log2 ratio of mean freqeuncies?

Hello al! I am looking to calculate and plot error (SD, SEM, etc) for comparing the change in frequency for many immune cell populations between TumorType1 vs TumorType2 (indolent breast tumor vs...

29 September 2020 8,332 1 View

How do I interpret an interaction between variables in a linear mixed model?

For example, I have a 2-level factor variable (diagnose) in interaction with a 6-level factor variable (layer). Intercept by default is the first level of all. In my case intercept is control...

18 September 2020 6,211 4 View

Is there a functional difference between a two-way ANOVA and a one-way ANOVA followed by Tukey's post-hoc?

Hi, I have been thinking about this for a couple months, and I am wondering if anyone else has any input. Lets say I have 4 treatments 1) 0 nM compound X + 0 nM Compound Y 2) 10 nM compound X + 0...

18 August 2020 8,583 5 View

Why do scRNAseq pipelines (e.g. seurat) have the filtering step to remove cells that express over 2500 genes? Meaning, why 2500?

Does anyone know how this number became "default"? what is it based on? How deep is our knowledge of "average" , "healthy" gene expression of different cell types? Sorry if my question is trivial...

04 August 2020 6,691 1 View

Has anyone had success with antibody conjugation kits for ICC/IHC studies?

I am planning a multiplex ICC experiment. The issue is that several of my antibodies are anti-rabbit which would cross react with secondary antibodies. I was wondering if anyone has used antibody...

23 March 2020 6,136 3 View

Is there another site which acts as a forum for device process engineers to discuss recipes/approaches/observations/tips/etc.?

I am well aware that recipes we may come across in a journal article or elsewhere rarely work properly in a different location with a different tool, however, it shows that a thing is possible and...

26 February 2020 2,827 5 View

How can I run and interpret a mixed effect model with a random factor in Stata?

Hi all, I have a set of data (attached) for which I need to run a mixed model with a group variable as a random factor. The data are grouped by the column titled "Group Variable". The group...

13 January 2020 9,398 5 View

Is anyone able to source a canadian either TLR 4 KO, TLR 2 KO, MyD88 KO, or IRF3 KO cell lines?

Hi, I am looking for immune cells with any of the above knocked out, before I look into a CRISPR system to do it myself. I have only been able to source mice with these genes knocked out, but I...

17 November 2019 1,957 2 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Tyler Hurst

Thomas, yes we have those on the way. What I'm interested in are recommendations for dilute acid solutions for soaking and length of soak times to prepare our new labware. The initial elements of interest are Al, Ca, Cr, Cu, Fe, Mn, Ni, K, Ti, Na, Mg and Zn. We are shooting for Detection Limits of

Khaled Muftah Elsherif

All glassware should be soaked in 10% nitric acid prepared from ultra pure aci

Thank you Khaled