Hi Everyone,

You see a 2D electrophoresis image of Royal jelly proteome profiling at the attachment. I wonder that can the use of chromatofocusing helps to get similar results?

If applicable, what kind of columns and the buffers should I use? Is the providing Mono P 5/200 GL from Cytiva and polybuffer exchangers such as PBE 118 and PBE 94 only way to do it with highest resolution?

Is there any more common, easier, and low-cost methodology to perform? For instance, would the charge variant analysis using WAX column with from high pH to low pH gradient or vice versa with WCX be sufficient?

Note: I'm aware of some proteins have same pI but different MWs, but I only pay regard to IEF separation. Co-elution of the proteins that have the same pI but different MWs is not a problem for the beginning of my research.

Thanks in advance for any suggestions...