Does anyone know the exact type of mutation (may be in PRKDC) which made CHO-V3 cells a DNA-PKcs deficient one?
How can we differentiate between calcite, dolomite, siderite, magnesite and ankerite minerals in carbonatite rocks in thin section under optical microscope?
07 August 2024 2,132 3 View
Hi, we have measured tryptic peptides using both DDA and DIA method on QExactive. In DDA replicates i saw unusual intensity drops occurring at the same sections of chromatograms in DDA replicates...
07 August 2024 3,218 4 View
Dear fellow researchers, I am currently working on a paper where I need to provide a reliable reference that defines and distinguishes between 3D mesh models and 3D city models. Although I am...
06 August 2024 9,986 2 View
Hello, Can anyone provide me with the absorption coefficient of methane gas at 7.7 um? Any reference?
06 August 2024 980 5 View
I am conducting a qualitative study that uses interviews to investigate the perceptions of teachers about a particular leadership practice and I am focusing on 3 schools which have a total number...
01 August 2024 8,457 10 View
Hello I am conducting a microstructural analysis of a soil treated with lime. The following sem images are of the untreated s1 and treated soil s3. The untreated soil contains quartz calcite...
01 August 2024 572 0 View
HI there, I've came across several articles discuss about random audit an Non random to tax evasion or compliance. Most of the articles is relating about effect of audit (random or non random)...
31 July 2024 5,309 7 View
Can we patent a process flow diagram developed using a process simulator but no actual cases is carried out? For example consider a process for certain product manufacture where a new process flow...
31 July 2024 781 1 View
How can we calculate the percentage of configuration interaction (CI) in the UV output data of the Gaussian program? for example: Excited State 17: Singlet-A 5.1359 eV 241.41 nm...
28 July 2024 9,165 2 View
Please, what is the memory consumption of the Matlab function quad tree decomposition procedure [S = qtdecomp(I)] with respect to the input set I?
27 July 2024 5,455 2 View
Hey! I aim to generate a transgenic knockin zebrafish line that mimetizes a genetic condtition that leads to a certain disease on human. To do so, I need to insert a codon for mutagenic aminoacid...
14 July 2024 6,240 0 View
Studying a specific monogenetic disease based on mutations in different individual genes. I try to recreate the disease phenotypes of donors in vitro. I knocked out a gene x with above 90%...
12 July 2024 6,313 0 View
I have done site-directed mutagenesis (Substitution) to obtain a Cysteine mutation in my gene of interest. Now, I have two domains in my protein, And I want to delete one of the domains to obtain...
05 June 2024 9,430 1 View
Now, I am using CRISPR/Cas 9 system to make a point mutation at THBD gene. I am wondering how can I isolate the desired cell population after making a point mutation by using CRISPR/Cas 9? Are...
30 May 2024 9,993 0 View
We want to suppress self proteolysis of a protein. What is the strategy to modify the sequence in order to make it stable? The site is SLG - NX and SLG - AX.
25 May 2024 7,649 0 View
I am using GraphPad prism for data visualisation and analysis. I am trying to assess if there is a statistically significant difference between the percentage of 2 cohorts (patients and cell...
17 May 2024 4,424 1 View
I am working on a project to detect genetic mutations in lung cancer using machine learning algorithms and I am new to genetics and medical sciences. Can I use RNA as input to the algorithm for...
13 May 2024 5,726 6 View
This is from an endomyocardial biopsy from a 24 year old male with clinical hypertrophic cardiomyopathy. However, no mutation is found in the related genes. What are the irregular nuclear-like...
12 May 2024 8,148 1 View
presently I'm doing one study on ASSOCIATION OF COMT GENE VARIANT WITH ALCOHOL USE DISORDER. the methodology consists of conventional pcr with comt primers and rflp with NIA III rest enzyme. after...
22 April 2024 4,927 3 View
Hi lentivirus experts, I have lentivirus plasmids that carry mutations in the 3' and 5' LTR region. These regions were not exactly designed for easy cloning. Therefore, I wonder if it is possible...
14 April 2024 3,054 2 View