I am planning to do a lot of ChIPs following 'Chromatin techniques for plant cells Bowler 2004' and thus I am planning on stockpiling a lot of stock solutions and premade ChIP buffers. I'm having a very difficult time finding any consistent info on storage conditions for even individual solutions and whether they should be sterile and whether to do it by autoclave or filter. Multiple sources give conflicting information so I've tried to compile what I felt was the consensus below.

I was wondering if I could get any help confirming that the below information is correct and fill in the missing info?

A: typical storage condition/time in storage

B: storable at -20C?/time stable at -20C

C: storable at -80C? /time stable at -80C

D: light sensitive?

E: should be autoclaved?

F: should be filter sterilized?

G: special considerations

Stock Solutions

Formaldehyde 37% :

A: RT/2 yr B: Yes/indefinite C: Yes/indefinite D: NO E: NO (obviously) F: NO G: hazardous

Glycine 2M :

A: 4C/2 yr B: Yes/indefinite C: Yes/indefinite D: NO E: YES (autoclave or filter sterilize) F: YES

SUCROSE 2M :

A: RT/6m B: Yes/indefinite C: Yes/indefinite D: NO E: YES (autoclave as long as solution does not turn brown or filter sterilize) F: YES

Tris-Hcl pH 8 1M :

A: RT/indefinite B: Yes/indefinite C: Yes/indefinite D: NO E: NO F: NO

B-ME 14.3M:

A: RT/? B: Yes/indefinite C: Yes/indefinite D: ? E: NO F: NO G: hazardous

PMSF 0.2M :

A: -20C/? B: Yes/indefinite C: Yes/indefinite D: NO E: NO F: NO G: hazardous

MgCl2 1M:

A: RT/indefinite B: Yes/indefinite C: Yes/indefinite D: NO E: NO F: NO

Triton X 20%:

A: RT/indefinite B: ? C: ? D: NO E: NO F: NO

EDTA 0.5M:

A: RT/indefinite B: Yes/indefinite C: Yes/indefinite D: NO E: NO F: NO

20% SDS

A: RT/indefinite B: No/precipitates C: No/precipitates D: NO E: NO F: NO

NaCl 5M:

A: RT/indefinite B: Yes/indefinite C: Yes/indefinite D: NO E: NO F: NO

NaHCO3 :

A: RT/indefinite B: Yes/indefinite C: Yes/indefinite D: NO E: NO F: NO

NP-40:

A: RT/indefinite B: Yes/indefinite C: Yes/indefinite D: NO E: NO F: NO

LiCl 4M: A: RT/indefinite B: Yes/indefinite C: Yes/indefinite D: NO E: NO F: NO ChIP Solutions Extraction Buffer 1 0.4 m Sucrose 10 mm Tris–HCl, pH 8.0 5 mM β-ME 0.1 

A: 4C/6m? B: Yes/indefinite C: Yes/indefinite D: NO E: NO F: YES

with sodium butyrate 5mM

A: 4C/6m? B: Yes/indefinite C: Yes/indefinite D: NO E: NO F: YES

Extraction Buffer 2 0.25 M Sucrose 10 mm Tris–HCl, pH 8.0 10 mM MgCl2 1% Triton X-100 5 mM β-ME

A: 4C/6m? B: Yes/indefinite C: Yes/indefinite D: NO E: NO F: YES

with sodium butyrate 5mM

A: 4C/6m? B: Yes/indefinite C: Yes/indefinite D: NO E: NO F: YES

Extraction Buffer 3 1.7 M Sucrose 10 mm Tris–HCl, pH 8.0 0.15% Triton X-100 2 mM MgCl2 5 mM BME

A: 4C/6m? B: Yes/indefinite C: Yes/indefinite D: NO E: NO F: YES

with sodium butyrate 5mM

A: 4C/6m? B: Yes/indefinite C: Yes/indefinite D: NO E: NO F: YES

Nuclei Lysis Buffer 50 mM Tris–HCl, pH 8.0 10 mM EDTA 1% SDS

A: RT/1yr? B: Yes/indefinite C: Yes/indefinite D: NO E: YES F: YES

with sodium butyrate 5mm

A: RT/1yr? B: Yes/indefinite C: Yes/indefinite D: NO E: YES F: YES

ChIP dilution buffer 1.1% Triton X-100 1.2 mM EDTA 16.7 mM Tris–HCl, pH 8.0 167 mM NaCl

A: RT/1yr? B: Yes/indefinite C: Yes/indefinite D: NO E: YES F: YES

with sodium butyrate 5mm

A: RT/1yr? B: Yes/indefinite C: Yes/indefinite D: NO E: YES F: YES Elution buffer 1% SDS 0.1 m NaHCO3

A: RT/4hr B: Yes/indefinite C: Yes/indefinite D: NO E: NO F: ? Low salt wash buffer 150 mM NaCl 0.1% SDS 1% Triton X-100 2 mm EDTA 20 mm Tris–HCl, pH 8.0

A: RT/indefinite B: Yes/indefinite C: Yes/indefinite D: NO E: ? F: ? High salt wash buffer 500 mM NaCl 0.1% SDS 1% Triton X-100 2 mm EDTA 20 mm Tris–HCl, pH 8.0

A: RT/indefinite B: Yes/indefinite C: Yes/indefinite D: NO E: ? F: ?

LiCl wash buffer 0.25 M LiCl 1% NP-40 1% sodium deoxycholate 1 mM EDTA 10 mm Tris–HCl, pH 8.0

A: RT/indefinite B: Yes/indefinite C: Yes/indefinite D: NO E: ? F: ?

TE buffer 10 mm Tris–HCl, pH 8.0 1 mM EDTA

A: RT/indefinite B: Yes/indefinite C: Yes/indefinite D: NO E: ? F: ?