8 Questions 2 Answers 0 Followers
Questions related from Ad Ad
I'm doing a 3C analysis shown in the picture linked and attached below with the Bait fragments labeled and the target fragments that will be tested for interaction with the bait labeled with...
03 June 2024 7,989 1 View
I am planning to do a lot of ChIPs following 'Chromatin techniques for plant cells Bowler 2004' and thus I am planning on stockpiling a lot of stock solutions and premade ChIP buffers. I'm having...
02 April 2022 1,030 1 View
I've done a few ChIPs so far for eventual sequencing and I've finally gotten through one where I don't remember making any obvious mistakes. I mostly follow these...
08 April 2019 6,089 3 View
04 April 2019 9,428 2 View
After some consulting I decided to use a commercial pcr cleanup spin column kit to concentrate DNA from my CHIP for sequencing. The DNA was in elutate of sodium bicarbonate and 1% SDS....
02 February 2019 7,422 2 View
Hello, I am planning a ChIPseq of Arabidopsis looking at a TF binding. My current plan is to do an induced inflorescence, uninduced inflorescence, and leaf tissue ChIP. I am generally following...
12 December 2018 2,166 2 View
I'm running an EMSA and I'm finding that adding additional protein causes the free probe band to start to disappear instead of shift up into a new band. It seemed to work before so I don't really...
26 July 2018 5,339 3 View
07 July 2018 10,047 4 View
