30 March 2022 2 9K Report

Hi,

I am looking forward to perform ChIP-qPCR for histones (e.g. H3K4me1, H3K27ac, etc). Can any one guide that how to make qPCR primers for ChIP analysis? I am aware that these are different from normal expression analysis PCR primers. I am little confused that if my target region is intronic, then how can I quantify my region of interest?

More Ali Faiq's questions See All
Similar questions and discussions