6 Questions 8 Answers 0 Followers
Questions related from Ali Faiq
Hi everyone, I am in search for positive and negative controls for some of Histone marks for ChIP-qPCR in HUDEP cells. Can anybody help in this matter? Marks are: H3K27ac H3K27me3 H3K4me3...
13 March 2023 5,824 2 View
Hi, I am looking forward to perform ChIP-qPCR for histones (e.g. H3K4me1, H3K27ac, etc). Can any one guide that how to make qPCR primers for ChIP analysis? I am aware that these are different from...
30 March 2022 9,357 2 View
Dear All, My protein is getting expressed in undouble form. I want to purify it in native form so I have not attached any tag. Can any one has experience and guide on how to purify protein from...
30 September 2021 4,628 5 View
I have downloaded BRIG0.95 and JAVA 8.0, but I am unable to open the BRIG.jar file with JAVA? Is that a version compatibility issue, if so, what are the versions that are compatible with each other?
13 July 2021 336 4 View
Hello everyone, Can the same efficiency be achieved from 0.1 cm and 0.2 cm electroporation cuvettes, if the parameters are kept constant? Anyone?
31 August 2020 2,707 6 View
Hello All, I have prepared a protein marker using proteins expressed by my lab. Now, I am interested in making a pre-stained marker and I need guidance for how to make a marker pre-stained using...
01 January 1970 1,570 4 View