How to use the bioluminescent ATP assay kit?

I need to do a mitochondrial ATP quantification assay in insects and for this, I have a bioluminescent ATP assay kit but I don't have a protocol yet. I'd like to know if you have some kind of...

24 January 2021 3,655 3 View

How to take advantage of the social isolation generated by COVID-19 to boost the transition from face-to-face education to remote education?

The isolation generated by the COVID-19 pandemic has forced most of the world's universities to choose remote or virtual classes. In the case of engineering programs and other programs where real...

28 March 2020 2,350 57 View

Is it possible to reduce energy consumption without affecting human well-being?

Climate change is a reality. Responsible for this change is the excessive concentration of CO2 in the atmosphere, mainly due to the requirement of electricity at the residential, commercial,...

13 February 2020 8,805 12 View

Western Blott results?

To publish a result of western blott it is necessary to show the bands of the total protein? In the case of not showing this part of the result, can it still be published?

20 March 2019 5,596 2 View

Help with troubled ligation-clonation experiment.

14 August 2017 2,881 13 View

Help with troubled ligation-clonation experiment.

07 August 2017 1,301 14 View

Are we misusing the P-value statistics in diversity studies? What options do we have to implement the hypothesis testing?

Hi everyone, I just read in Nature a warning for the researchers who are attempting to test a hypothesis: the ASA "American Statistical Association" explains that is concerned by the wrong use of...

08 March 2016 3,775 4 View

Which rhBMP-2 ELISA Kit is recommendable to use in the detection of the protein in culture medium: R&DQuantikineELISA Kit or CUSABIOELISA Kit?

I need to measure the release of rhBMP-2 in cell culture medium from immobilization of the protein in a scaffold and I have only the two options. Thanks in advance.

07 September 2014 1,661 3 View

No title

I tried it with long incubation times (3 days for primary antibodies), 0,3% Triton, heat-, enzymatic- or citrate buffer-induced antigen retrieval but it doesn't work. Any advice about the protocol?

11 December 2012 3,260 19 View

No title

I tried it with long incubation times (3 days for primary antibodies), 0,3% Triton, heat-, enzymatic- or citrate buffer-induced antigen retrieval but it doesn't work. Any advice about the protocol?

06 December 2012 9,701 19 View

Can Ethovision store the generated data and video recordings directly to NAS system?

The size of data generated by Ethovision is huge. Having NAS system for data storage seems to be a good option, I am wondering whether Ethovision can directly store the data to the NAS system...

27 February 2021 6,350 2 View

Does anyone have a stack of images of action potentials or abnormal brain scans?

I need a stack of black and white .jpeg/.tiff/.jpg/.png images across time of either action potentials, neurons firing, or brain scans (comparing disease and normal brain, disease progression,...

09 February 2021 2,648 3 View

How to increase bacterial concentration of DNA from Laser capture microdissection samples?

I used laser capture microdissection, selected specific bacteria presented in ethanol-fixed slides. And DNA was extracted by Qiamp mini kit. The problem right now is we have pretty low...

09 February 2021 3,908 2 View

What can I do to keep magnetic beads suspended in solution within a syringe?

I'm looking for ways to prevent the settling of magnetic beads in solution. I have been using a syringe pump to inject a suspension of magnetic beads in PBS into a microfluidic device, and so far...

09 February 2021 6,206 2 View

Are there any fast-acting, long-term fixatives compatible with FISH?

I'm looking for a fixative that works quickly but you can safely store cells in for a longer period (i.e. a few hours). Most importantly it must be able to fix chromatin well and be compatible...

03 February 2021 7,659 7 View

Help needed: How to map AA, AG, and GG genotypes on to polymorphism information?

Dear Colleagues, I am new to genes and genotyping and I need help. I am trying to figure our how AA, AG, and GG genotypes map on to A1 for DRD2/ANKK1 Taq1A polymorphism(rs1800497) and Val or MET...

02 February 2021 3,861 3 View

Is it better to push or pull liquid through microfluidics with a syringe pump?

I have a PDMS microfluidic device that I want to perfuse cells through (there are also adherent cells in the channels). Is there any difference if I use a syringe pump to push the cells through...

30 January 2021 4,092 3 View

How can you attach polyurethane devices on glass slides?

I am making microfluidic devices made of polyurethane. The problem I am facing is that I am unable to attach these devices to glass slide even after plasma treatment (air plasma). Some of the...

28 January 2021 5,194 2 View

Does the pre-synaptic neuron location in a cortical map influences the spatial position of its synapses in the dendrite of the downstream neuron?

Are functional maps in the cortex used by the brain to carry out computations or are they just a byproduct of wiring minimization? A key element to answer this question is to know if, when...

26 January 2021 1,118 8 View

Lighting for open field test behavior assay?

I am currently setting up our lab's behavioral space to run the open field test in rats, and I am trying to figure out how to set up lighting to evenly illuminate the open field chamber (a 90 cm x...

26 January 2021 1,399 3 View