How feasible would it be to perform ChIP RT-PCR from small samples (no more than a few hundred micrograms) of brain tissue? Does anyone have any protocol/suggestion/experience with this?
I thinks there is one nature protocol named micro ChIP would help you very much.
take a look at the methods in this paper: http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0029758
Chromatrap have a kit that allows you to perform ChIP using as little as 500ng of chromatin
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The main differences
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