Dear colleagues, fellow scientists and students,
I am working on establishing of protocol for BioID in plants. I am aware of already existing protocols such as cell culture, the transient transformation of tobaccos and root culture. For our purposes, we will need to work with seedlings and I was wondering if someone ever tried to perhaps transfer the seedling onto solid MS media enhanced with biotin. Could that work? What would be a good concentration to start with? I'd be grateful for any insights or links to relevant literature backing up the yes-no argument.
Thank you and all the best in your research!
Hi! I transfer 7-10 day Arabidopsis seedlings to MS liquid media + 40 µM Biotin for with smooth shaking some hs and works perfectly (For TurboID). I find this much easier than transfering them to solid media, specialy for a large amount of plants.
Good luck with you experiment! :)
Tomás María Tessi Thank you for your input!
That is exactly what we wanted to do initially however we need to do a treatment to plants that should be 24-48h and I do not think the plants will survive it. Or they would be so stressed that the whole experiment will be biased.
So I am thinking about alternative methods. But perhaps I can do the treatment and transfer them to the liquid MS+biotin as biotinylation itself is 2-6h.
Thank you and good luck in your research too, maybe I just needed to look at it from different angle :)
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