If I have a 40% chimeric male mouse, how many matings would be needed to get germline transmission to F1? Is there a breeding calculator which provides this information?
What component or agent can the TE buffer contribute to having an unsuccessful PCR result
12 June 2024 6,574 7 View
The journal "The transition from gel separatory serum tubes to lithium heparin gel tubes in the clinical laboratory" aims to assess the viability of replacing serum with plasma samples in...
08 April 2024 3,341 3 View
In a study titled "Diagnostic Efficacy of Serological Antibody Detection Tests for Hepatitis Delta Virus: A Systematic Review and Meta-Analysis" by Zhenzhen et al., findings indicate that although...
08 April 2024 1,247 1 View
overall strategy and analytical approach that you have chosen in order to integrate, in a coherent and logical way, the different components of the study, thus ensuring that the research problem...
01 April 2024 9,676 1 View
I've been using the Qiagen Circulating extraction kit to extract the viral RNA from plasma samples. Further I used an in-house RT step (very similar to SuperScript IV from invitrogen) to get cDNA....
12 March 2024 10,022 0 View
This question is posted as it was stated in the study "Diagnostic Microbiology from the Beginning to the Future: Regional Antibiograms as Public Health Tools to Slow Down Antibiotic Resistance"...
05 February 2024 3,038 2 View
Hi, I'm in the process of developing a hydrolysis probe qPCR assay to quantify gene copy numbers of Microcystis 16S rDNA. In troubleshooting my cycling parameters, I'm getting resulting plots such...
15 January 2024 6,957 1 View
I am conducting an exergoeconomic analysis of HVAC system energy consumption in a classroom, considering three parametric cases: air conditioning only, air conditioning with mechanical...
14 November 2023 133 3 View
In 2012, there were 78 million new instances of gonorrhea among adults, making it the second most prevalent bacterial STI in the world. Infertility, pelvic inflammatory disease, ectopic pregnancy,...
01 September 2023 252 1 View
Hi all, please assist me on what statistical analysis I should use. I have 3 DVs (severity of violence, justification of violence and severity of punishment) and 1 IV (gender of perpetrator). I...
21 August 2023 5,311 3 View
I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?
11 August 2024 3,115 1 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
Hello dear colleagues, We have prepared a manuscript on NiTi-based alloys and are seeking a second opinion on our current TEM results. If you are a Ph.D. holder with experience in TEM and have...
07 August 2024 9,563 0 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....
04 August 2024 762 1 View
average particle size calculation from TEM
04 August 2024 2,921 1 View
I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...
31 July 2024 9,186 0 View