For attachment of OLIGO Nucleotides...Is there any chemical method to activate polycarbonate surface other than plasma and UV treatment?
Dear All, I am working on Rapid test development (Lateral flow based). I am facing conjugate release issue after few months of strip preparation. Up to approx 30 days, conjugates release found...
20 July 2023 8,648 8 View
Hello All, I am working on E. coli and want to study its gene expression profile using qPCR. I grew the culture till stationary phase and snap freeze the cell pellet using liquid nitrogen. I...
20 April 2023 7,280 4 View
I am trying to express the soluble fraction Membrane Protein SARS Cov2 in E. Coli. I am getting only monomers while it's the dimer form that is active. Literature that I have come across have used...
20 March 2023 4,459 1 View
Hi, I'm looking for a clean and direct visualization method to image dsDNA (assume purified extract available) with a fluorescence microscope. Currently, the most common ways to "visualize"...
16 January 2023 4,525 1 View
I'm constructing a phylogenetic tree using microsatellite sequences in MEGA11. I have aligned the sequences using MUSCLE and now I can see a lot of "xxx" and gaps in many of the sequences. Kindly...
13 September 2022 6,517 3 View
Is using BIO-RAD Total protein extraction kit for plant total protein extraction a good decision ?
26 August 2022 9,344 3 View
I'm looking for approximate cost just to include in the funding proposal. Due to short timeline I couldn't get quotation from companies.
17 August 2022 1,004 4 View
Dear All, I am working with E. coli and want to determine its fatty acid content using GC-MS. I have converted fatty acids into FAMEs by chloroform/methanol method and have my samples in...
02 August 2022 5,102 3 View
As shown in the figure, I have a design with rings of a parabolic reflector, each segment with a different thickness. To do this, I created a parabola in one work plane, a circle (guide curve) in...
01 March 2022 4,282 1 View
I just need the explanation or basic examples to show
25 November 2021 1,475 4 View
A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...
11 August 2024 9,801 2 View
Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!
11 August 2024 3,770 4 View
A website software of Blackbody radiation law expert software can used through the following web site. http://39.105.188.151:3000/index
07 August 2024 1,706 0 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
I'm researching light sources and their UV emissions. I'm curious if a 50W, 7500K conventional LED lamp emits any UV light. I found that a 200W tungsten lamp can emit up to 1.5 mW/cm² at 350 nm....
31 July 2024 3,843 7 View
I have different polymer solutions and I hypothesize that water molecules are retained based on the water-polymer interactions, so the amount of free water is less to intercat with drug tablet....
28 July 2024 9,545 6 View
How can we calculate the percentage of configuration interaction (CI) in the UV output data of the Gaussian program? for example: Excited State 17: Singlet-A 5.1359 eV 241.41 nm...
28 July 2024 9,165 2 View
I was performing estimation of Total Soluble Sugar content in bacterial cultures by Anthrone method. Out of 9 samples, only one sample showed positive reading while others had a negative reading...
22 July 2024 9,576 4 View
i need to find ECC for 4-MIMO antenna using radiation pattern on CST
16 July 2024 7,291 1 View
I am a beginner with ImageJ and would like to know how to quantify fluorescence images of platelets taken with a fluorescence microscope at 40x and 100x magnification. If you could provide...
11 July 2024 1,464 7 View