Based on the statistical comparison of spectrophotometric and chromatographic data, the interchangeability between HPLC and UV spectrophotometric techniques has been suggested for the routine analysis.
Thank you.
It depends if you have interferences in your sample that absorb in the wavelength of choice and the sensitivity of both methods.
You are wandering into a 'minefield'! A UV scan only gives you the absorbance at a particular wavelength, while HPLC/PDA can do that plus a retention time match (relative selective retention based on a particular mobile phase and column). Many antibiotics have many different isomers which have many different activities, thus many different peaks). A method that works well on the dry drug substance, may not work on the finished drug form (FDF), or clinical samples (like blood or urine). Thus, a UV scan can give you a false positive results on a clinical sample even though there are NO antibiotics present!
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