Conventionally, in a polymerase chain reaction (PCR), oligonucleotide primers bind to the template DNA in an antiparallel complementary way and the template DNA is amplified as it is. Here we describe an approach in which the first primer binds in a parallel complementary orientation to the single-stranded DNA, leading to synthesis in a parallel direction. Further reactions happened in a conventional way leading to the synthesis of PCR product having polarity opposite to the template used. This is the first study showing that synthesis of DNA can happen also in a parallel direction. We report that from a single-stranded DNA template, two different but related PCR products can be synthesized.
Article Parallel DNA polymerase chain reaction: Synthesis of two dif...
Congratulations for your study, but I am actually confused, how is the DNA backbone arranged in this case, the hydrogen bonds formed between A and T as well as between G and C, can only work when the strands are anti-parallel.
Is it a valid question or am I just confused? 5'to 3' will not fit with 5' to 3', even when they have complementary bases.
Thanks Simran Bhullar but to remove your confusion , you can read full paper and reference within manuscript.
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