What comes first
1. RNA
2. Single stranded DNA
3. Double stranded Parallel DNA
4. Double stranded Anti parallel DNA
Check out
http://f1000research.com/articles/3-320/v1
ds DNA (PCR product) obtained in lane (A) (which run at 600 bp) was used as template DNA and using primers which binds Internally to (A) ds DNA PCR product B (674 bp) and ds DNA Product C (713...
01 February 2017 8,286 11 View
You may read my views at http://f1000research.com/articles/4-438/v1
07 August 2016 1,842 2 View
Villain of Molecular Biology: Why are we not reproducible in research? http://f1000research.com/articles/4-438/v1#ref-37 Towards reproducible...
08 September 2015 1,966 7 View
http://m.f1000research.com/articles/3-320/v1
03 April 2015 6,118 0 View
Conventionally, in a polymerase chain reaction (PCR), oligonucleotide primers bind to the template DNA in an antiparallel complementary way and the template DNA is amplified as it is. Here we...
01 February 2015 6,886 2 View
01 February 2015 8,878 13 View
Dr. Kary Mullis received a Nobel Prize in chemistry in 1993, for his invention of the polymerase chain reaction (PCR). The process, which Kary Mullis conceptualized in 1983, is hailed as one of...
31 December 2014 9,588 0 View
Approximate concentrations are require in compared with the WHO permissible limts
11 August 2024 2,723 1 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,539 5 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
Hi, I'm currently working on a project where I need to plot the atom-projected band structure using GPAW. I've been able to calculate the band structure for my material, but I'm having trouble...
07 August 2024 269 3 View
Hi all, my experimental XPS results shown that my C3N5 sample consists of N-H bond, hence in this case I should incorporate the N-H bond into my DFT modelling. However, I do notice several papers...
07 August 2024 8,414 2 View
Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...
06 August 2024 807 3 View
I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...
06 August 2024 1,367 3 View
Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...
06 August 2024 1,495 2 View