Hey Sadiye, it would be more accurate to check both (forward and reverse) primers (the product) when checking primer specificity. It would be a real problem if the primer pair is not specific, not just one primer.
Without the reverse primer sequence it is not feasible to design a matching forward primer, because primer annealing temperature, complementary regions, etc. need to be determined to select an appropriate pair with a high probability of efficient amplification.
We use a universal revers primer.
I have design this 2 forward primer for my sequence are they good?
1) my original: GGTGGCTGTAGCTCAGTTGGTAGAGTCCAGGATTG
My forward primer: GTTGGTAGAGTCCAGGATTG
2) my original :CGCGGGGTGGAGCAGTCTGGCAGCTCGTCGGGCTCATA
my forward primer: GCAGTCTGGCAGCTCGTC
Which gene from which species are you going to amplify?
As mentioned several times above, please provide the sequence of the reverse primer without that it is impossible to determine whether the primer combination might work.
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