Can anyone help me this question?
Normally, in cell culture, we add FBS in RPMI media. However, in case that we add our materials containing electrostatic or hydrophobic interactions into the cell solution. There is possibility that proteins in medium will be absorbed. How can we know that the ONLY nanoparticles were taken up or as a clusters (nanomaterials + proteins absorbed)
Thank you very much
The hypothesis that either - only protein intake takes place or NP intake!
the third option might be taken up as clusters but in all the cases one thing is sure there must be ease of competitiveness. In the following experiment you need to evaluate all these 4 conditions along with control that none has been taken up (cell culture evade).
Media standardization before and after the experiment may be one of the way, similarly many more such experimentation can be set.
Since the problem discussed in short so it is not easy to comment much.
thanks
Hello Thanh,
I suggest to focus on method(s) to detect/identify the NP on the sample, which I think is the main objective. if NP were adsorbed successfully then there are various assays for total protein such as BCA, lowrey, UV.
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