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Can anyone help me this question? Normally, in cell culture, we add FBS in RPMI media. However, in case that we add our materials containing electrostatic or hydrophobic interactions into the cell...
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There is a research project regarding detection of H. pylori in gastric biopsy samples, so anyone has done it, can you please help? After using a gastric biopsy sample for RUT-CLOtest, can the...
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Hi everyone I have been doing RNA clean-up using Qiagen kit, however running out of spin-columns. Any recommendations or advice on how to get new columns without purchasing the kit? Thanks lots.
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Hi everyone, ethanol resists in my samples after RNA extraction by TRIzol and purification by RNeasy Kit (Qiagen), which makes it very difficult to load on gel. Has anyone experienced the same...
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I'm having a problem, Ethidium Bromide keeps running out of my gel, which makes the bands become very faint. Any suggestions to troubleshoot this? I ran 90V, for 30 mins. 1.5% agarose in TBE buffer.
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