Cell numbers in powder increase again after decreasing?

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I did not get nice tight bands for my RNA extraction. Anyone can suggest me pls?

I do not understand why I did not get nice two bands.

17 June 2024 783 2 View

How to convert ppm to micromoles per gram FW for Trehalose measurement from plants?

I would like to know how to convert ppm to micromoles per gram FW for trehalose measurement. I'm using the method described by by Li et al. (2014). Li, Z.-G., Luo, L.-J., & Zhu, L.-P. (2014)....

17 April 2024 4,038 2 View

I wrongly posted my research paper with other's topic. How can I change my research topic?

My paper is uploaded but the topic is not agree with my research paper. I don't know why?

16 September 2023 342 2 View

How much sample is needed to ignore for assumption of normality? According to central limit theorem, what does large enough sample mean?

I am a Ph.D nursing student. I am analyzing my data, 845 samples for normality test. I found that my dependent variable and independent variables are not normal distribution. According to central...

02 February 2023 8,656 6 View

Where and how is GHQ-12 available?

I wonder where and how is General Health Questionnaire (GHQ-12) available. Is it free or do I need to purchase it? Thanks in advance ...

13 January 2023 8,442 0 View

Do you have a procedure for making a standard calibration curve of Acetaldehyde by GCMS?

Hello, I would like to make a standard calibration curve for Acetaldehyde by GCMS in order to analyze the content of Acetaldehyde in rPET samples. We have been trying several ways. However we...

21 June 2022 6,305 4 View

How we can test the traits or quality of an entrepreneur in a controlled environment?

I think, determination is the most influencing trait of an entrepreneur, how we can assess this quality in controlled environment.

06 April 2022 8,224 2 View

How to calculate species richness, diversity index, etc. by using Ranger Patrol data?

Within the protected areas, the rangers recorded any tracks and signs of animals while patrolling. They recorded any tracks and signs of animals, location, times and identified species by using...

14 November 2021 8,459 3 View

How should I do article search for a review?

I am collecting articles to do review as research. Instead of searching in databases, is it acceptable to search in a certain number of discipline-specific journals with high impact factors?

12 October 2021 5,557 10 View

Can we measure Acetaldehyde by only GCMS without FID and headspace sampler?

Dear All, I would like to measure Acetaldehyde and limonene content in recycled polyethylene terephthalate (rPET). AA can be measured by GC-FID with headspace sampler and limonene by GC-MS....

23 August 2021 4,547 12 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

To perform transfection with DharmaFECT Duo in AGS cells. Could you tell me what the ideal concentration is to avoid significant cytotoxicity?

I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...

03 August 2024 3,851 1 View

Can i quantify a nanoparticle encapsulated in cell culture medium by hplc?

Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...

03 August 2024 2,730 1 View