How can I differentiate full structural equation model (measurement model + structural model) from (just) path analysis?

Even in some high impact journals, there are articles in which - there is no description that a variable is a latent (unobserved) one, - the researcher(s) do(es) not report the statistical method...

23 January 2020 612 3 View

Does someone know how to co-stain foxp3 and phospho-protein for flow cytometry analysis?

Now I'm trying to analyze phosphorylation of signal molecules in Treg. To do that, initially I chose foxp3 staining kit from e-bioscience. But most of cytosolic proteins were gone, although foxp3...

26 August 2016 2,244 12 View

What kind of ANGPTL4 antibody is suitable for Apoptome?

I have to work on ANGPTL4 expression in lung cancer by using Apoptome immunofluoresence microscope. I have no experience in IF and I have difficult in choosing primary and secondary antibodies...

15 June 2016 5,793 1 View

How shall I develop measurement items for an acceptance study using Technology Acceptance Model?

Most of the technology acceptance studies adapt measurement items from previous validated questionnaires. How shall I develop a measurement for a latent construct which has almost never been...

13 October 2015 1,314 7 View

What is the best diagnosis for these sections of liver ?

hello every one, can any one ANNOTATE these sections ?? with best histopathological diagnosis ? I have some notes but i want the best annotations, Because i am new in this disciplinary !

01 April 2015 2,871 6 View

How do you generate a dendrogram from repetitive element PCR?

I would like to know how to generate a dendrogram from repetitive element PCR profile. Please see the attached publication. If somebody knows how to do that, please teach me.

22 August 2014 9,796 3 View

Is there any acceptable R2 value for educational research in PLS-SEM?

I am doing PLS-SEM.According to Joseph F. Hair and his colleagues, the acceptable level of R2 depends on the research context with higher values indicate higher level of predictive accuracy. In...

01 January 1970 8,531 4 View

What should I do if high cross loadings between PU and BI indicators in TAM?

I did SEM for my TAM model and found high cross loadings between PU and BI indicators. However, factor loadings on respective constructs are higher than cross loadings and Fornell-Larker criterion...

01 January 1970 1,542 2 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

What is the most reliable source to get Impact factor of journals?

Source to get Impact factor of journals

01 March 2021 6,654 2 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View

Which index or sites are reliable in determining of plant species?

There are many indexes when determining plant species: Plants of the World Online (POWO), International Plants Names Index (www.ipni.org), The Plant List (www.theplantlist.org) and others. But...

26 February 2021 3,404 10 View