I would like to know how to convert ppm to micromoles per gram FW for trehalose measurement. I'm using the method described by by Li et al. (2014).
Li, Z.-G., Luo, L.-J., & Zhu, L.-P. (2014). Involvement of trehalose in hydrogen sulfide donor sodium hydrosulfide-induced the acquisition of heat tolerance in maize (Zea mays L.) seedlings. Botanical Studies , 55(1), 20.
Raghad Mouhamad
Example Calculation:Let’s assume you have a trehalose concentration of 478 ppm. The molar mass of trehalose ((C_{12}H_{22}O_{11})) is approximately 342.3 g/mol. If your sample’s fresh weight is 1 gram:[ \text{Micromoles per gram FW} = \frac{478 \times 342.3}{1000} = 163.1 , \mu mol/g ] Interpretation:Your trehalose concentration is approximately 163.1 micromoles per gram fresh weight.
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