Cell line contamination?

02 July 2025 1 3K Report

Has anyone ever seen contamination that is white and seems to form a starfish similar shape. It is resitant to Ampicillin, Gentamicin and Hygromycin B? How do I kill it?

Erik Moore

This is a fungal contamination. To resolve the issue I washed the cells thrice in PBS and treated with 25 ug/ml of Amphotericin B. Even though this is a very high concentraion it did not bother my cells.

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