I have never done a minimal time course, but in my hands an overnight (12 to 16h) stimulation with LPS (1µg/ml) of MACS purified B cells (B220 positive selection) always resulted in nice upregulation of CD80 and CD86 (or CD54 and MHCII). The data/ protocol you find here: Article MYSM1-dependent checkpoints in B cell lineage differentiatio...
I have never done a minimal time course, but in my hands an overnight (12 to 16h) stimulation with LPS (1µg/ml) of MACS purified B cells (B220 positive selection) always resulted in nice upregulation of CD80 and CD86 (or CD54 and MHCII). The data/ protocol you find here: Article MYSM1-dependent checkpoints in B cell lineage differentiatio...