Can I use a categorical covariate in a Generalized Linear Model in SPSS v.26?

Hello everyone, hope you are well. I am planning to use a Generalized Linear Model in SPSS. I would like to add gender to the model as a covariate, however from what I have read, only continuous...

14 February 2021 8,937 4 View

One-Way or Two-Way ANOVA?

Dear, In the preparations for publishing a manuscript, we have a big argument about the proper analysis of variance to compare multiple groups for a certain staining quantification. We have two...

27 December 2020 7,916 3 View

Is there a substitute to InhibitEX buffer in Qiagen Stool DNA extraction Kit?

I have a DNA extraction kit and would like to know if there is a way to make my own InhibitEX buffer (or something similar with the same function) to remove PCR inhibitors commonly present in...

23 August 2020 3,079 2 View

I need to perform antioxidant testings on Histocyctic lymphoma cells, any suggestons of another cell line, if it doesn't become available?

I need to perform antioxidant testings on Histocyctic lymphoma cells, any suggestons of another cell line, if it doesn't become available?

02 August 2020 4,502 2 View

As an academic researcher in Arab universities, what is your impression about the experience of distance education in light of the Corona 2020 ????

As an academic researcher in Arab universities, what is your impression about the experience of distance education in light of the Corona 2020 ????

04 May 2020 3,099 3 View

Can PLAXIS 3D use to evaluate the liquefaction analysis?

Dear researchers, I would like to know the ability of PLAXIS 3D, which can use to evaluate the liquefaction analysis or not? If can, could you please provide me links of its relating tutorials...

25 July 2019 7,058 3 View

DNA extraction from dry sealed tissue?

Hi, my colleague stored his samples of Drosophila pupae in PCR plate. He just put samples in wells, sealed it with PCR foil and left in room temperature for couple of months. He did not add any...

14 April 2019 3,282 2 View

What can be the potential applications of alloy powders?

I want to complete my research work by studying properties and applications of alloy powders.

17 April 2018 5,529 5 View

Is it possible to buy normal plasma cells from healthy donors or RNA at least?

Hi, I need normal plasma cells or RNA as control for myeloma plasma cells. A years ago it was possible to buy them from AllCells but I could not find it. Thanks a lot. Hana

14 February 2018 3,282 4 View

Any suggestions for desalting tips (or columns) to purify protein samples before gel top analysis (looking for high cut-off products 100-200 KD)?

high salt concentration in my sample is causing smearing in SDS-PAGE with no clear bands

21 November 2016 7,950 7 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

What degree of missing data in a particular case/participant results in Expectation-Maximisation no longer working?

I'm dealing with a mediation model and am using the PROCESS module in SPSS. Due to SPSS and PROCESS being limited in the imputation methods - being unable to handle multiple imputation - the other...

02 March 2021 4,362 1 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View