I am doing multiplex immunofluorescence assay on FFPE section. I used CD3 to identify T cells and observed that some of T cells stained with CD3 showed nuclear staining instead of membranous. I am not sure why the staining pattern is nuclear for CD3? is it possible to see nuclear staining with CD3?
From which tissues are your FFPE section?
In pro-thymocytes initial expression of CD3 occurs in the cytoplasm in a peri-nuclear location. During T-cell maturation, cytoplasmic CD3 expression is lost and the CD3 antigen is found on the cell surface.
In T-cell neoplasms (e.g. T-cell lymphomas, T-cell acute lymphoblastic leukemia) CD3 may be cytoplasmic.
thanks so much . Yes the tissue is FFpE. Do we expect this expression pattern for some cells in FFPe cancer patient samples?
This depends on the type of cancer you are investigating and the tissue from which the FFPE sections are made. As mentioned, e.g. T-cell lymphomas show cytoplasmic CD3 staining.
Try to figure out in which tissue/cell type you see the cytoplasmic CD3 staining.
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