CAT activity of fermented extract using human RBC lysate?

10 October 2017 0 1K Report

I have used 40 µL (2 µL RBC lysate (2:10000) in 67 mM PBS (pH 7.4)), 40 µL fermented/unfermented extract and incubated for 15 min at 37 ⁰C. And then add 15 mM H2O2 and measured the ansorbance at 240 nm for 2 min. But after 2 min ELISA reader gave reading overflow. But I can not understand what the problem?

And What is the formula for measuring catalase activity?

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