How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Hello fellow researcher .. What is the meaning of your name?

I would like to create a small guide containing the meanings of the names .. Thanks to everyone who cooperates with me in achieving it.

03 March 2021 5,266 4 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Should the AUC in a concentration-time graph of a subcutaneous implant be roughly the same for poeple?

I have 2 concentration time curves of a subcut drug implant. the first group has a peak around the 50 day mark where as the second group does not. Both groups received the same implant by the same...

10 February 2021 2,305 3 View

Separated objects vs Non-Manifold Assembly?

I'm trying to do a simulation between the Maxilla and the Mandible I converted the Dicom file from the CT scan to a CAD module and module the articular discs via 3d software. I have two options...

08 February 2021 8,731 2 View

How to perform CV by using potentiostat?

I have deposited a metal oxide thin film of glass plate, A conductive thin film was also deposited on the glass substrate before the deposition of metal oxide film. How can I perform its CV from a...

01 February 2021 7,710 3 View

Is TRIS-EDTA-CITRATE buffer (pH 7.8) a good antigen retrieve for the TUNEL assay?

Hello everyone! I performed a TUNEL assay simultaneously with an immunofluorescence (SOX9 antibody) on the same sections of human testis. The fluorescence of TUNEL turned out well, but that of...

15 December 2020 1,270 3 View

Hello, How long can rat sperm survive in saline solution after leaving from the testis after sacrification?

How should I measure motility, what is the process? Should I put it in the incubator (37 C) immediately after sacrification? Thank you from now.

28 October 2020 280 4 View

What is the Standard Operating Procedure(SOP) for anthocyanin extraction from red rice?

Anthocyanins are rich in antioxidant properties. Previous research has shown that red rice contains a good amount of anthocyanins. While there are a number of laboratory procedures for anthocyanin...

24 August 2020 5,159 7 View

Tissue Digestion with proteinase k gives "invisible" globule"?

I'm digesting a few different mammalian tissues with a spin kit and proteinase k. Some tissues (esp. testis and pituitary gland) look fully digested and clear. But once the lysis buffer is added,...

09 August 2020 4,443 2 View

Can I use a spin column kit to extract DNA from bull testis?

I'm extracting DNA from various bull tissues and there are some that don't work with a spin column kit, like brain tissue that contains a lot of lipids. For those, we do Phenol-chloroform...

05 August 2020 2,126 2 View

How to tell or calculate group difference in subgroup meta-analysis?

Dear all, For some time, we have been working with Stata metaprop in order to analyse our results from our review on two surgical techniques (for now A and B). Through the metaprop command...

03 August 2020 4,693 1 View

Enriching Operating Room (OR) Based Student Learning: How Should We Structure it? What models are appropriate for medical students?

Various models like Apprenticeship model, BID model, Koen's Model, One Minute Preceptor (OMP), 4C/ID model etc have been previously utilized for surgical resident's training. Dynamics of student...

29 July 2020 3,308 3 View