What common set of parameters do you usually include when determining the soil quality index in your locale?

I want to understand better the usage of parameters on soil quality index determination.

06 January 2021 974 6 View

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What are the technicalities involved ? What are the reasons ? Can we share some facts on this

31 December 2020 5,698 5 View

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31 December 2020 6,159 4 View

Don't we think use of non-functional drugs in different Nations led to genome re-engineering by Sarscov-2 virus to survive,& turns to variants ?

Re-engineering of genome my Sarscov-2 and emergence of variants now in UK, USA, South Africa, Nigeria and now upcoming in other countries.. What are the key facts and reasons ? Thank you...

31 December 2020 745 4 View

What is the upper and lower limit for plate count for fungi?

I am currently trying to quantify the viable count of trichoderma spp. in a compost sample. However I do not have much literature on the range for plate count of fungi. should I be following the...

19 December 2020 8,874 6 View

Is the "Releve" method still standard protocol for plant community sampling and percent cover estimation?

I am not a botanist but need to estimate the average percent vegetation cover of my study area. I have researched this and found articles that use the "releve" method for determining plot size...

22 September 2020 5,676 6 View

What colour does your Pd(PPh3)4 catalyst have during and after C-C coupling. Especially in Stille-reaction?

Hello, I am working on a C-C coupling of a strong electron accepting aryl-cl and Bu3Sn-Benzonitrile. I was able to synthesize my product but the yield is very low. I always have a big residue/...

10 August 2020 6,762 8 View

Compare varying parameters with limited experimental setups?

Hello colleagues, I am currently working on solar desalination experiments. I have to find the optimum angle at which my solar still gives maximum performance. But I only have two solar stills and...

15 March 2020 6,416 4 View

How to measure the inside temperature of the glass in a solar still, without the effect of irradiation of sun on the thermocouple?

I am building a solar desalination still and the inside glass temperature is required for the condition whether the glass has attained the dew point temperature of the vapor inside the still. I am...

09 January 2020 4,435 3 View

Is any data available as to the angular dependence of Young's modulus in carbon/epoxy 2x2 twills?

22 December 2019 9,073 1 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Should GFP sequence be cut together with the IRES sequence?

Hello. I have a viral plasmid which contains a bicistronic region: CMVprmtr-GFP-IRES-PuroR. I would like to cut out the GFP sequence to get rid of the green fluorescence. In this case, would it be...

02 February 2021 1,644 7 View

How to do DNA normalization based on qPCR values?

Hi Everyone: I have a question regarding to qPCR. I am trying to do RT-PCR, and I want to have equal amount of input DNA for my RT-PCR, so I normalize my DNA input based on qPCR values of a...

31 January 2021 6,977 4 View

How to gate mouse MDSC cells through flow cytometry?

On inducing the mouse bone marrow cells with X compound, there is a change in cells morphology and characteristics. In that case how to gate these cells, as these cells are showing suppressive...

27 January 2021 608 2 View

Is it possible for Cre positive mice to be negative for flox reporter gene (eGFP)?

I was genotyping some mice tail snips. It is a Cre Lox reporter mouse. The Cre is expressed using CRH promoter in the mouse brain and eGFP is the reporter gene. I isolated g-DNA and used it for...

12 January 2021 6,875 3 View

What can be the reason of having high Cq-values for reference genes?

Hi, Once again I am facing an annoying problem. This time in my barley qPCR, I am trying to detect a virus (BYDV) in plant samples. I get rather high Cq-values for 2 of the 3 reference genes...

06 January 2021 4,685 9 View

Loss of reporter gene expression from lentiviral transfer plasmid?

We have produced a 3rd generation, self-inactivating lentiviral vector for the expression of our gene of interest under the EIF1alpha promoter. This vector also contains the GFP reporter gene...

22 December 2020 4,918 3 View

Can forward and reverse sequences of PCR products be entirely different from each other?

Dear All, The forward and reverse sequences obtained from the PCR products are not identical at all. Can I still make the contigs for doing BLASTn? Individually both sequences are showing the...

22 December 2020 5,280 5 View

Is the expression of House keeping genes disturbed in an abnormal situation like in a diseased tissue?

In gene expression studies, what would be the reliability of house keeping genes expression say in a disease/insect attacked tissue that is being subjected to studies (RT qPCR ). Will the...

14 December 2020 3,445 5 View

Normalisation of qPCR between two different species?

If I have a gene carried on a plasmid and the plasmid is transformed into two strains of different bacterial species (Salmonella and e. coli). If I want to do RT-qPCR to quantify the expression of...

03 December 2020 8,051 5 View