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I am learning MD simulations using GROMACS and while I was doing a protein in water MD simulation a doubt raised. I was following the steps mentioned on the tutorials page and it said The system...
15 February 2021 5,514 4 View
In 2D Model, Reaction in porous Zone, Error occurs on hooking UDF , DEFINE_VR_RATE in Fluent, .The error spikes just after running calculation after 1st iteration. Divergence detected in AMG...
16 November 2020 9,053 3 View
We have 2 machines that test products. Both of these qualify the product to be Good or Bad ( Based on amount of leak ). The final outcome is attribute variable (Good/ Bad) whereas the way we...
16 November 2020 688 7 View
Need to create 3d surface plot. Data I have X, Y ,Z coordinate of the pipe surface and the corresponding Q (heat flux) values at that point. Nearly 222440 data points of a 2m length pipe. Thank you
07 October 2020 9,854 7 View
Looking towards an incompressible flow (with K-e, turbulence model), with mass flow/velocity inlet and Pressure outlet boundary condition. Which initialization method preferred? How far it can...
05 October 2020 4,742 5 View
They say we were just speaking and not writing anything for tens of thousands of years till a basic form of writing evolved just about ten thousand years back. Why this yawning gap ?
22 September 2020 5,313 7 View
ANSYS or ABAQUS donot correlate materials chemistry wirh the observed or simulated mechanical properties. Is there a software that can do this structure to property correlation. ?
22 September 2020 1,171 3 View
I am currently pursuing my research in Phycology. I'd like to know if there's any simple and cost-effective method to detect and analyze nutrients and vitamins in the algal matter. The experiment...
18 September 2020 4,081 3 View
I am currently pursuing my research in Phycology and planning to conduct a study on microalgae. Is there any place where I can send my samples to be identified? (Preferably in South India) Also,...
17 September 2020 4,440 2 View
Hi, I am working on molecular validation of co-expressed genes in Rice under moisture stress. I have a list of genes that are co-expressed in three rice varieties, under a particular set of...
12 July 2020 7,494 1 View
Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?
03 March 2021 1,763 2 View
Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...
03 March 2021 1,499 2 View
Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...
03 March 2021 6,299 3 View
i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity
03 March 2021 8,890 1 View
dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...
03 March 2021 6,994 5 View
Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...
01 March 2021 2,215 2 View
Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.
01 March 2021 9,952 3 View
I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...
01 March 2021 9,310 4 View
I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...
01 March 2021 9,015 2 View
Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...
01 March 2021 2,622 3 View
