The following review article provides details of several chemical and biological mineralization methods and their comparison under submerged rice soils.
Nitrogen availability indexes for submerged rice soils by K. L. Sahrawat Advances in Agronomy vol. 36 p415.
Yes, there are alternative methods to analyze mineralizable nitrogen (N) besides the potassium permanganate method. Some common methods include:
Anaerobic Incubation: This method involves anaerobically incubating soil samples for a set period to stimulate microbial activity and mineralization of organic nitrogen compounds. After incubation, mineralizable nitrogen is determined by measuring the increase in ammonium-N concentration.
Aerobic Incubation: Similar to anaerobic incubation, this method involves aerobic incubation of soil samples to stimulate microbial activity. After incubation, mineralizable nitrogen is determined by measuring the increase in nitrate-N concentration.
Chloroform Fumigation-Extraction: This method involves fumigating soil samples with chloroform to kill soil microbes and halt biological activity. After fumigation, the difference in extractable ammonium-N or nitrate-N concentrations between fumigated and unfumigated samples provides an estimation of mineralizable nitrogen.
Biological Activity Assays: These assays measure microbial respiration or other indicators of biological activity in soil samples as proxies for mineralizable nitrogen. Examples include measuring carbon dioxide evolution or ATP production.
Isotopic Tracer Techniques: Isotopic tracer techniques involve adding isotopically labeled nitrogen compounds (e.g., 15N-labeled organic matter) to soil samples and tracking the release of labeled nitrogen as it mineralizes over time. This method provides insights into the dynamics of nitrogen mineralization and microbial nitrogen transformations.
Soil Enzyme Activities: Certain soil enzymes, such as urease and protease, are involved in the mineralization of organic nitrogen compounds. Measuring the activity of these enzymes in soil samples can provide information about potential rates of nitrogen mineralization.
Note: Each of these methods has its advantages and limitations, and the choice of method may depend on factors such as your objectives, soil characteristics, and available resources. It's important to consider the suitability and accuracy of the chosen method for the intended application.