I have just bought the 25mg bottle and I want to resuspend it all at a specific concentration. However the documentation is vague on the specific solubility on DMSO and I would also like to have a sensible stock concentration.
Hello Eva,
you could prepare a 25 mM TMRE stock solution (e. g., dissolve 12.8 mg TMRE in 1 ml ethanol (p. a. quality)). I would recommend to store this stock at -80°C.
Best regards, Christian
Hello Eva,
you could prepare a 25 mM TMRE stock solution (e. g., dissolve 12.8 mg TMRE in 1 ml ethanol (p. a. quality)). I would recommend to store this stock at -80°C.
Best regards, Christian
Hi Christian,
Many thanks. However the documentation says to dissolve in DMSO. Is there a difference?
Thanks
Hello Eva, I think there is not much difference whether you use absolute ethanol or DMSO for preparing the TMRE stock solution. Both solvents should work fine.
Regards, Christian
Jordan, I have used TMRE in the past and remember making 1mM stock in DMSO and stored at -20 degree C. Hope this helps!!!
- Badges
- Science topic
- Similar topics
- Biological Science
- Molecular Biology
- Molecular Biological Techniques