To launch PhyML ?

07 August 2019 1,282 3 View

How can I download Seqstate software ?

I aligned IGS1 sequences via MEGA software but there are a lot of indels in the dataset. I should use Seqstate program (Indelcoder) for recodes the gaps. However I didn't download this software...

11 December 2018 1,201 2 View

Why I saw smear band in the agarose gel?

I amplified fungus DNA and run in the 1% agarose gel. I prepared agarose gel with Gelred dye. However, I saw smear bands. What is the reason of this smear bands? I give amount of pcr reagents...

06 July 2018 8,163 21 View

Why did I see this yellow phase on the top layer during DNA isolation chloroform isoamilalcol step?

I extracted genomic DNA from macrofungi with CTAB method. In the chloroform step, I saw a yellow layer on the top layer (see pictures, I showed it with number 3). What is it the reason for this? I...

06 July 2018 9,807 4 View

How can I align IGS sequences?

I am working on phylogeny of fungi. I amplify IGS1 region but I can not aligned IGS sequences because there are lots of indels. Which programme can I use for aligned and phylogeny? How should I...

04 May 2018 9,667 5 View

Why my sequences are bad?

I am working on phylogeny of fungi. I amplify ITS region and get sequences. However the sequences are consistently bad nowadays. I didn't resolve this problem? I can not find the cause of the bad...

04 May 2018 2,782 6 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to upload pdf of my articles published?

How to upload pdf of my articles published?

03 March 2021 2,183 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View