To launch PhyML ?

07 August 2019 1,282 3 View

Why I saw smear band in the agarose gel?

I amplified fungus DNA and run in the 1% agarose gel. I prepared agarose gel with Gelred dye. However, I saw smear bands. What is the reason of this smear bands? I give amount of pcr reagents...

06 July 2018 8,163 21 View

Why did I see this yellow phase on the top layer during DNA isolation chloroform isoamilalcol step?

I extracted genomic DNA from macrofungi with CTAB method. In the chloroform step, I saw a yellow layer on the top layer (see pictures, I showed it with number 3). What is it the reason for this? I...

06 July 2018 9,807 4 View

How can I align IGS sequences?

I am working on phylogeny of fungi. I amplify IGS1 region but I can not aligned IGS sequences because there are lots of indels. Which programme can I use for aligned and phylogeny? How should I...

04 May 2018 9,667 5 View

Why my sequences are bad?

I am working on phylogeny of fungi. I amplify ITS region and get sequences. However the sequences are consistently bad nowadays. I didn't resolve this problem? I can not find the cause of the bad...

04 May 2018 2,782 6 View

Can you suggest me a sci-exp journal which scopes molecular biology and fungus taxonomy, please?

I am looking for a journal for my master paper. The scope of my paper is molecular biology and fungus taxonomy. I think, my paper can be published a sci-exp journal. So, I need your suggest..

02 March 2018 9,360 3 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

What degree of missing data in a particular case/participant results in Expectation-Maximisation no longer working?

I'm dealing with a mediation model and am using the PROCESS module in SPSS. Due to SPSS and PROCESS being limited in the imputation methods - being unable to handle multiple imputation - the other...

02 March 2021 4,362 1 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View