Can you suggest a good method for colony PCR for Aspergillus sp.?

Daniela Pasanisi @Daniela-Pasanisi

23 February 2016 1 2K Report

I've tried different methods but they didn't work. Do I need a kit?

Thank you

http://www.fgsc.net/aspergillus/spore-pcr2.pdf

http://www.aspergillus.org.uk/sites/default/files/pictures/Lab_protocols/AspergillusColonyPCRProtocol.pdf

Rafik Karaman

Dear Daniela,

You may try one of the following:

1-http://www0.nih.go.jp/JJID/62/164.pdf

2-http://www.ncbi.nlm.nih.gov/pubmed/16110805

3-http://www.ncbi.nlm.nih.gov/pubmed/9519482

4-http://jcm.asm.org/content/40/8/2860.full

5-http://publicationslist.org/data/torsten-bohn/ref-5/Pallez_FdTEchBiotech_2014.pdf

6-Colony Multiplex-Tandem PCR for Rapid, Accurate Identification of

Fungal Cultures

http://www.ausdiagnostics.com/qilan/Assets/JCM4058-60%20Dec%202008.pdf

For using kits:

Direct colony PCR of several medically important fungi using Ampdirect plus.

http://www.ncbi.nlm.nih.gov/pubmed/19305063

Hoping this will be helpful,

Rafik

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