Can you remove samples from TEM grids?

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Brent E Gowen

Easy to answer this one...NO!

Can you not just cut more sections from the block?

Denis Korneev

Theoretically, it is possible to find some conditions, when the resin slices detach from the film. For example, it could be a gentle sonic treatment in warm water or something similar. But it will take lots of time to develop such a protocol, and it still won't be reliable. But why do you need it?

Joel Turner

Brent E Gowen thank you for your condescension.

Denis Korneev The reason is, why should I keep purchasing grids if I can reuse them...

I might try your sonication method and report back!

Vadym Chibrikov

Good day, Joel Turner! Hope you are doing well.

I am not an expert on that field, however, I found some promising sources that may provide you necessary info. Please, check the following links:

https://marclab.org/tools/protocols/grid-cleaning-protocol/

https://iubemcenter.indiana.edu/equipment/tips-and-help/removing-plastic.html

Article Formvar Assisted Graphene Transfer for Graphene TEM Grid

Feel free to mention me in your response in case of any extra questions.

Best of luck in your research!

Yours sincerely,

M. Sc. Vadym Chibrikov

Department of Microstructure and Mechanics of Biomaterials

Institute of Agrophysics, Polish Academy of Sciences

ok...your question was less than clear. Can you reuse grids ?...yes. This is routinely done in EM labs. Can you do this by cleaning off old sections while keeping the formvar film and carbon...No.

Don't believe me, spends years trying to achieve that than finding out how EM labs clean their grids, make new Formvar films and carbon coat them! You're the expert.

Okay...... If my question was that unclear then you ask to clarify. I'm simply a PhD student trying to learn here.

For reusing the answer is NO. The reason is simple - even if you will remove the slices, the film likely will be damaged/contaminated, thus you'll just spend your and TEM's time for nothing. But grids can be reused, just wash them with organic solvent (acetone, dichloroethene, etc), and then coat with formvar.

Good luck!