Can you recommend me some protocols for alkylation of MGDGD, DGDG and SQDG?

More Dag Ekeberg's questions See All

How to select the best model in multi-level approaches?

Hi, I am trying to fit growth functions on several levels of a fish population (global, by habitat, by size at maturation) by non-linear mixed effects models (since the age/length data originates...

24 September 2019 7,380 2 View

Is the bigger LRI (Linear retention index) or RI (retention index) means bigger concentration?

is the bigger LRI (Linear retention index) or RI (retention index) means bigger concentration or peak area or percentage of peak area for GC MS measurements?

16 June 2017 3,071 5 View

Can anyone suggest me a measurement tool to collect data to identify student leadership perception of teachers at schools?

I need urgent help in data collecting for my research in educational leadership to identify student leadership perception of teachers at schools. The measurement tool can be quantitive or...

12 May 2015 8,067 3 View

Why is my plasma cell detection with CD138 IH not 100% consistent and CD56 is not working at all?

For bone marrow trephines we use a glutaraldehyd fixative, which for internal reasons is imperative. Decalcification in EDTA has been changes from overnight at 35°C to a stirred bath at 45°C, but...

08 February 2015 8,850 10 View

Is there any research going on substitutes of current synthetic corticosteroids?

I was wondering if, due to high imbalance with dosages in long term illnesses of adrenal gland(like Primal Adrenal deficiency) and broad spectrum of side effects (especially hydrocortisone)...

26 November 2014 7,337 4 View

Where can I find a good SSE-vectorized Smith-Waterman implementation in C?

I need to do pairwise alignment of nucleotide sequences of length 15-200 nt. Preferably, it should be written in C and accept *char variables for the sequences.

26 June 2013 5,853 3 View

Can you recommend a method to isolate (preparative) monoglycreides from diglycerides and from triglycerides?

We work with lipids in milk and usually I prefer using SPE. Can you recommend other methods to isolate (preparative) monoglycreides from diglycerides and from triglycerides?

01 January 2013 5,765 5 View

Amber20 step by step procedure ?

How I can used AmberTools 20 (all steps) to make Molecular Dynamics Simulations for carbohydrates and lipids like i have a mixture of both glycolipids and phopholipids.

06 September 2023 3,512 0 View

What are the differences between glycolipid & proteoglycan?

Is Glycolipid or proteoglycan same thing?

12 February 2023 7,616 1 View

Can I use Anti-Glycolipid Antibodies (human source ) in case of bovines?

I am working on bovine glycolipids and am unable to find out ready-to-use anti-glycolipid antibodies from bovine sources which further leaves one option i.e. customizing bovine-specific antibodies...

09 February 2023 125 0 View

Finding the collaborator who can help to generate data for small-Angle X-ray scattering of liposome samples?

Hello everyone, This is Sayan. I am a graduate student in UF, chemistry. In one of our project, we want to validate one conclusion regarding the unique clustering behavior of some...

22 December 2022 8,466 0 View

What is the Criteria to select glycolipid and phospholipid candidates from LC-MS data?

I have generated LC-MS lipidomics data from mammalian germ cells. Out of the data, I couldn't find any glycolipids and very few phospholipids and most of the data contains metabolites. I would...

29 August 2022 9,025 4 View

Can anybody suggest me a better protocol for lipid extraction from bacteria sample (Like as Acinetobacter baumannii) ?

After TEM analysis I got my mutant outer membrane have some rough surface. So I want to extract lipid from my mutant.

09 October 2019 5,453 3 View

LC-MS: same compound, 2 RT?

Hi, I have an issues with the detection of a standard on LC-MS. I run MGDG standard (prepared in methanol, LC-MS grade) on HILIC BEH Amide column; the run is 15 minutes long, using a gradient...

16 June 2019 4,476 4 View

LC-MS: same compound, 2 RT?

Hi, I have an issues with the detection of a standard on LC-MS. I run MGDG standard (prepared in methanol, LC-MS grade) on HILIC BEH Amide column; the run is 15 minutes long, using a gradient...

05 June 2019 1,784 5 View

What is Electronic Potential Energy Density?

Greetings everyone! I am doing a research on disaccharide glycolipid, a sugar molecule. I did optimization and frequency calculation on the sugar molecule via Gaussian. In order to discover the...

09 October 2018 8,861 4 View

Can partition coefficient always predict the elution volume in HSCCC experiment?

Hello there, I am developing a HSCCC (high-speed counter current chromatography) protocol to separate glycolipids using a system of butanol-acetonitrile-MTBE-water. I added a small portion of...

09 October 2018 7,651 1 View

Ana Reis

Dear Dag,

I guess when you wrote MGDGD you meant MGDG, right?

MGDG, DGDG and SQDG glycolipids contain a lipid moiety and a sugar moiety. Alkylation of sugar moiety can be done by several procedures, exactly those used in carbohydrate chemistry. You might want to try and search the one most suitable for your needs and purpose.

My experience with derivatization of (sugar) carbohydrates involves acetylation with acetic anhydride in mild acidic conditions. The mild acidic conditions are not strong enough to hydrolyse the sugar residues, and the yield of derivatization reaction is complete. It is a fairly easy and straightforward procedure. My advice is for you to try the procedure with less valuable sample before implementing it to your glycolipids.

Hope this helps,

Ana

Dag Ekeberg

thank you for your answer! I would also suspect that was a ok way to go, so I will try as you recommend!