What is the Criteria to select glycolipid and phospholipid candidates from LC-MS data?

How to compare different concentrations of nanoparticles for characterization from ICP-MS?

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I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

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How does the injection volume impact LLOQ and ULOQ in regression analysis (LC-MSMS)?

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Why ICP-MS test for Teflon filters that collected PM2.5, show really high results in the millions of ug/g ?

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What influences inter-day reproducibility in UPLC-MS/MS analysis?

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How do I prepare and store insect samples for JH measurements?

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MS/MS protein analysis?

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2.6 Drying gas flow controller cannot regulate the requested setting after a fixed amount of time?

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How to distinguish pure and adulterated honey using NMR spectroscopy?

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20 January 2021 5,456 12 View

Mohammad Tajik

Hi Fanny,

The most recommended lipid extraction method is the Folch method. You can find the details in the following manuscript:

Article Lipid Extraction by Methyl-Tert-Butyl Ether for High-Through...

I don't know which lipid annotation software did you use, but I recommend the Lipid Search Software if you have the access to that. In this software, you can modify the search details and select phospholipids or glycolipids.

Hope you can get the best results.

Regards.

Kiran kumar Kukkala

I do encountered a similar problem and I have tried using Lipid Search software or lipid maps or lipid suit with the same LCMS spectra to identify the different types of lipids.

Fanny Josan

Thank You Mohammad Tajik for the answer.

I did outsourcing and they used LIPIDMAPS as the LIPIDSEARCH software is discontinued by Thermo (India) as per their statement. The data I got is not properly annotated and has maximum metabolites and very rare lipid molecules. I tried using LipidLynxX in LIPIDMAPS to annotate and it identified very less lipids from the total data. Now, I'm skeptical if I should ask them to re-analyze the data.

Also, Can I analyze it myself if I have raw files? If so, How?

Hii Kiran kumar Kukkala

Thank you for the answer.

I have tried using LipidMaps but the problem is the data I got is having a maximum of metabolites and very few lipids. I will definitely try Lipid Suite as per your suggestion but the data is unannotated. Is there any way I can annotate data and categories them into different lipid classes all at once?