Eunseo Yeo

6 Questions 3 Answers 0 Followers

Questions related from Eunseo Yeo

How to extract RNA from neurons?

Hi, I am a master's student who studies inflammation. I'm planning to extract RNA from the Vagal nerve or Dorsal Ganglion roots, but I got a question. It would be only a few 50ug per each nerve,...

24 July 2024 6,758 1 View

Is there any good protocols for dissect VG nerve?

Hi, I'm a student who studies neuroimmunology. I want to analyze mouse vagus nerve with RT-qPCR like this thesis but I don't know how to dissect the vagus nerve I found some protocol videos for...

11 June 2024 2,067 1 View

How do you keep tissue cold in tissuelyser ll when you extract RNA from animal tissue?

Hi, I'm a new master's student who has been studying mouse IPF model, and I read that Trizol with tissue needs to be cold. But I can't keep the tissue cold while I homogenizing it with...

31 May 2024 8,556 3 View

Could you advice me about rapid sample freezing with LN2, Isopentane?

Hi, I am a fresh master's student who just started to study Idiopathic Pulmonary fibrosis(IPF), and I am here to ask about how to freeze lungs with LN2 when I harvested lungs from mice because...

04 April 2024 2,190 1 View

Can you recommend me a good antibody for IL-6 Western blot or RT-PCR?

Hi, I am an MS student who is studying idiopathic Pulmonary fibrosis(IPF) My P.I asked to analyze cytokine IL-6 or TNF-a through Western blot despite Many researchers using ELISA for it because of...

27 March 2024 5,546 3 View

Is there any method to check Vagus nerve's activity with staining or markers?

I have been studying about Vagus nerve and I want to check the activity of the Vagus nerve in my research. Could you advice me about it?

18 March 2024 8,121 1 View