Can you recommend a cell line alternative to retinal ganglion cells?

More Robert Edgington's questions See All

Sampling size for unknown population?

Lets say I am sampling from an non-normal, multivariate population of unknown size and distribution. I'd like to know when I can stop sampling from the population (to a certain error) and have a...

08 September 2018 1,256 6 View

TAE buffer vs TE buffer for DNA stock storage?

Hi, Can DNA stocks be resuspended and stored in 1x TAE buffer vs. 1x TE buffer without negative effects? It would be simpler for my process to store the DNAs in TAE, however most sources recommend...

01 February 2015 9,208 0 View

Matlab, Keithley 2410, Current vs time?

Hi, I'd like to apply a fixed constant voltage using a Keithley 2410 and read out the current with respect to time using Matlab and SCPI commands via a USB/RS232 connector. I am using MaxOS,...

11 December 2014 7,021 2 View

Does anyone share a simple script using Keithley 2400 with Matlab?

Hi I'm trying to do a simple script that measures current vs time for a fixed voltage using a Keithley 2400. Does anyone have a simple script they could share on how to do this? So far I am able...

08 September 2014 2,788 1 View

What is the best Theory / Basis Set for graphene simulations in Gaussian 09?

I'm trying to do g09 simulations to obtain raman spectra of nanographene flakes (~ 24 carbon atoms) doped with nitrogen. Can you suggest a theory / basis set to use? Are diffuse orbitals...

05 June 2014 7,810 8 View

What is a good test (e.g. colormetric) for aminooxy groups (R-ONH2)?

I have seen Canaline and Jaffe's test, but I am not sure this is what I am looking for.

05 June 2014 552 2 View

Can gaussian09 be used to simulate valence band spectra?

And if so how?

02 March 2014 2,454 3 View

Any advice for impedance matrix construction?

I'm trying to construct a 2-terminal impedance matrix as described in the link. My network has multiple meshes. For non diagonal Zij elements where meshes do not share borders, what should the...

10 November 2013 3,794 3 View

Geotechnical Engineering (Proceedings of the ICE) time review?

Hello everyone, I recently submitted an article to Geotechnical Engineering (Proceedings of the ICE), and the current status has been listed as "EiC Pre-assessment: Ready" for the past 20 days. I...

10 August 2024 6,493 1 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

How to use Density Functional Theory to calculate carrier mobilities of solid system?

Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...

04 August 2024 8,894 1 View

Amritlal Mandal

You may try JAM-B Cells for your purpose.

Robert Edgington

Thanks. Are these non primary?

JAM-B cells (also known as J-RGCs) are a molecularly defined subcategory of direction-selective retinal ganglion cells that were first found in mice.

I guess the cells are primary. Not very much sure!!!!

http://wiki.eyewire.org/en/JAM-B_Cell