I am looking for a device that will perform automated multiplex immunofluorescence in frozen tissue and fluorescently label 4-5 markers on a single slide at the same time. Does anyone know which devices are better in this regard and have experience with them?
Dear Melike Sever Bahcekapili
These links might be useful, have a look:
1. https://www.youtube.com/watch?v=7xO7ste12U0
2. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7170662/
3. https://www.jove.com/t/58390/automated-multiplex-immunofluorescence-panel-for-immuno-oncology
4. https://jitc.bmj.com/content/8/1/e000155
Kind Regards
Qamar Ul Islam
I have good experiences with the Roche Benchmark Discovery platform. You can now stain up to 7 markers on ffpe or frozen sections. Its a open software machine, so u can work with any reagens you prefer. One of the nice things is that it uses flat incubation method and has options to add antibodies by hand if neccesary if dilution is too low. You can use this machine fully automatic for 30 slides at once.
I would be interested if you decided for a system. Most platforms seem to aim for many more than just 5 antibodies per slide and may therefore not be cost-effective. There is now a good selection of instruments for "ultra" high multiplexing and I was wondering if you found something that did the job for you that is more low multiplexing or whether you decided to perform an all manual multiplexing.
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