Does anyone have a detailed protocol for paraffin embedding of rat spinal cord?

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How can we create a hyperglycemic medium for MCF-7 breast cancer cell line?

In our lab, we already use "CAPRICORN high-glucose DMEM" (4.5 g/L glucose) for normal MCF-7-related experiments.

27 October 2023 4,206 3 View

Can you provide detailed information and suggestions about Automated Multiplex Immunofluorescence devices?

I am looking for a device that will perform automated multiplex immunofluorescence in frozen tissue and fluorescently label 4-5 markers on a single slide at the same time. Does anyone know which...

28 July 2021 7,608 5 View

Is there anyone using CELLINK BIO X for 3D skin bioprinting?

Is there anyone using CELLINK BIO X for 3D skin bioprinting? Can you provide advantage/disadvantages of this brand? Would you recommend this brand?

17 June 2018 5,630 3 View

How to conjugate carboxylic acid functionalized CdTe Qds with thiolated oligonucleotides?

Hi, I would like to modify the surface of carboxylic acid functionalized CdTe Qds with thiolated oligonucleotides. Can anyone help me for protocol to create thiolester bond between (CdTe...

04 October 2016 4,189 1 View

Is it a common practice that researchers use q. experimental and sequential explan. design in a single research ie, quan+qual then pre+post test ?

first quant. (scales and surveys) and qual (life story from control and exp groups) after that intervention (pre+ post control group design). any advantages + disadvantages ? Anyone can elaborate...

07 May 2016 4,625 6 View

How can I encapsulate ICG in Kolliphor HS 15 micelles?

Can I use solvent evaporation/thin film hydration to form the loaded micelles? Also, I've read filtration would be ineffective in separating micelles from free ICG because free ICG would bind to...

01 February 2016 737 1 View

How is 6-OHDA solution prepared for in vitro cytotoxicity studies?

To induce cytotoxic effect to SH-SY5Y cells, I want to use 6-OHDA, how can I prepare 6-OHDA solution to add medium of the cells?

21 May 2015 2,049 2 View

How can I prevent paraffin-embedded rat brain tissue sections from falling/coming off slides in H&E staining?

Paraffin-embedded rat brain sections fall off slides during H&E staining, especially transferring from 70% EtOH to water before hematoxylin staining. I used two different positively charged...

06 January 2015 5,131 25 View

What is the optimum time required for rat brain fixation after trans-cardiac perfusion?

For H&E staining and immunohistochemistry studies of brain tissue, we performed trans-cardiac perfusion with 4 % formaldehyde and then post-fixing in 4% formaldehdye for 24 hours. But I could...

24 December 2014 5,367 8 View

Is it enough to differentiate SH-SY5Y cells with 10uM retinoic acid treatment for 7 days?

I try to optimize human BIII-tubulin and GAP43 primers, so I want to differentiate SH-SY5Y cells into cells with morphological and biochemical characteristics of mature neurons. Therefore, is it...

04 December 2014 5,424 6 View

Do I need specific antibodies to stain for a fusion protein?

I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...

07 August 2024 5,338 2 View

Microtome tissue sectioning - How to prevent chatter of paraffin tissue specimen sections?

Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...

06 August 2024 666 4 View

How to determine positive-stained cells in FACS? Use isotype or unstained control?

To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...

06 August 2024 6,728 6 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,710 1 View

E-commerce businesses, COVID-19 and Entrepreneurial Strategies (innovation, proactiveness and risk-taking)?

Hello: I am looking for articles that address e-commerce businesses and COVID-19 survivors, as well as Entrepreneurial strategies that reflect e-commerce businesses. Blessings Evelyn Dempsey

05 August 2024 5,332 3 View

How to increase citation in Research Gate?

How to enhanced h-index in Research Gate?

04 August 2024 3,368 4 View

Senescence-associated beta galactosidase staining is False Positive in control group?

Hi guys If anyone is currently working on aging cells, you guys would like to give me some advice. I'm testing against biomarker (SA-beta-Gal), I encountered a false positive in the control group...

02 August 2024 6,735 1 View

Suggestions for the Annexin V Positive Control ?

Hello everyone, I am currently using washed human platelets to stain Annexin V as a procoagulant marker. Additionally, I am staining with PerCP-CD61 to identify platelet cells. So far, I have...

29 July 2024 8,624 1 View

How can I get clearer background for my western blot?

I am running a western on HIV envelope protein. The size is around 50Kda and I am using rat anti-HA for the first stain and a second stain of mouse anti-CA. Primary antibody dilution is 1: 1000...

29 July 2024 6,484 5 View

H-index issues?

Hi Sir/Madam, I would like to know why my h-index is stuck to 15 despite the number of new publications added. Could you explain me how marks are granted. Best, Daniel

28 July 2024 2,607 2 View