Which identification method is the most suitable or identification of Vibrio spp.? Molecular or Biochemical?

I have some Vibrio isolates from Brackishwater fish and environment. I am unable to identify two of them unto species level. It is showing conflict between Biochemical identification and Molecular...

03 January 2021 691 7 View

How to code in Matlab Ellipse by Oblong method?

How to code in Matlab Ellipse by Oblong method? The Video link is given below- https://www.youtube.com/watch?v=TNQE9vN3QJo&t=23s

08 November 2019 3,238 1 View

What is the difference between dark JV characteristics in solar cell vs. JV characteristics of OLED?

Dark JV characteristics of solar cell vs. JV characteristics of OLED - can they be analyzed in same way or same model?

26 July 2019 4,827 2 View

Is there any way to measure critical energy release rate of bonded materials solely in ANSYS?

Hi, I'm trying to figure out if there is any definite way to measure critical energy release rate only using simulation software like ANSYS. The Main reason behind using only Numerical method is...

17 July 2019 6,832 3 View

Which primer pair is the best for amplifying 16S rRNA gene of bacteria for identification purpose?

I was trying to amplify 16S rRNA gene of my Lactic acid bacteria (LAB) isolates. However, some commonly used primers failed to amplify some of my LAB isolates. Can anybody suggest the best primer...

06 May 2018 8,335 16 View

What does the raw variance-covariance ascii data file contain in GROMACS ?

I performed Principal Component Analysis (PCA) using GROMACS 5.1.3 with the following command gmx covar -f filename.xtc -s su2-now.gro -n protein-H.ndx -o eigval.xvg -v eigenvec.trr -ascii...

15 August 2016 8,022 8 View

Why is the combination of nose-hoover thermostat and MTTK barostat not working during NPT equilibriation of a protein in GROMACS ?

I tried to perform NPT equilibriation on a protein with nose-hoover thermostat and MTTK barostat with the following .mdp file ; VARIOUS PREPROCESSING OPTIONStitle = NPT simulation (constant...

11 July 2016 1,759 8 View

How to modulate velocity rescaling in gromacs during heating of a protein ?

I was trying to heat my protein system from 0 K to 300 K using the following temperature coupling tcoupl = v-rescale ; modified Berendsen thermostattc-grps = system ; one coupling grouptau_t = 0.1...

31 May 2016 7,956 16 View

How can I run a MD simulation in gromacs keeping protein heavy atom fixed ?

01 May 2016 6,517 4 View

How to perform SEM analysis using lavaan and R-studio software?

Dear all, I am trying to apply SEM (for the first time) to my graduate research, and I have been learning about SEM and lavaan from online resources and examples, but when it comes to my own...

16 November 2015 4,916 7 View

How can I assign a velocity along one direction to CO2 molecules in a molecular dynamics simulation using GROMACS?

Dear all, I am trying to perform a MD simulation about the interaction between CO2 and a polymer. I create my model, solvate it and perform the NVT and NPT equilibration. Now I am not able to...

26 February 2021 10,014 3 View

Recalcitrant seed short-mid storage method?

Dear peer scientists, Ultradry storage is not suitable for recalcitrant seeds. However, theoretically, if we can stabilize the structure of the subcellular membrane, as well as glassify the...

25 February 2021 2,442 1 View

Is there a suitable way to confine cell attachment area inside a multi-well plate?

Are there any types of cylindrical cell culture inserts that are designed to confine cell seeding area? Say, if I'm using a 12 well plate (3.5 cm^2/well), but only want to seed cells in the middle...

22 February 2021 7,127 4 View

Is it possible to use maize mature seed to do transformation for maize transgenic study?

Almost all research reports which i ever seen work on Early mature embryo for Agrobacterium mediated maize transformation. In other grass families like rice, we can use mature seed and what do...

17 February 2021 1,434 13 View

Dealing with BMDM seeding and RNA extraction ?

biomedical master student here , i am dealing with mice BMDM and would like some help , i seed 0.5 x 10^6 cells/per well in a 6 well plate but when i extract the RNA i get a very low yeild , i...

15 February 2021 1,102 5 View

Is secondary metabolite production more at vegetative growth stage or during reproductive stage?

Hi, I am working on the effect of heavy metals on a medicinal plant by pot experimentation. Analyzing the effect of heavy metals on secondary metabolites is one of the parameters. I will be...

11 February 2021 6,683 6 View

Are the topics below viable research in plant biotechnology?

1. Chemically induced mutagenesis in seed and qPCR detection and amplification of desired trait. 2. Site-directed mutagenesis in qualitative traits of a multiline seed.

04 February 2021 9,069 3 View

Seeds harvest from white clover by selfing?

I tried to get seeds from white clover by self-fertilization but I could not get the seeds. As you know the white clover is generally fertilized by crossing. I wanna get the seeds by...

04 February 2021 9,152 4 View

What is the best method of preparing cell supernatant samples for a Luminex assay to detect cytokines ?

I am planning to run a Luminex assay using cell supernatant to assess cytokine secretion by tumor cells. I came across different methods in literature; some suggest seeding in complete media and...

03 February 2021 4,867 3 View

How do I measure Arabidopsis germination rate?

Hi there, I'm looking for a way to measure the Arabidopsis germination rate, like what media I should use to plant the seeds and what indicator I should see for germination. I'm planning to...

02 February 2021 4,785 3 View