Which identification method is the most suitable or identification of Vibrio spp.? Molecular or Biochemical?

I have some Vibrio isolates from Brackishwater fish and environment. I am unable to identify two of them unto species level. It is showing conflict between Biochemical identification and Molecular...

03 January 2021 691 7 View

How to code in Matlab Ellipse by Oblong method?

How to code in Matlab Ellipse by Oblong method? The Video link is given below- https://www.youtube.com/watch?v=TNQE9vN3QJo&t=23s

08 November 2019 3,238 1 View

What is the difference between dark JV characteristics in solar cell vs. JV characteristics of OLED?

Dark JV characteristics of solar cell vs. JV characteristics of OLED - can they be analyzed in same way or same model?

26 July 2019 4,827 2 View

Is there any way to measure critical energy release rate of bonded materials solely in ANSYS?

Hi, I'm trying to figure out if there is any definite way to measure critical energy release rate only using simulation software like ANSYS. The Main reason behind using only Numerical method is...

17 July 2019 6,832 3 View

Which primer pair is the best for amplifying 16S rRNA gene of bacteria for identification purpose?

I was trying to amplify 16S rRNA gene of my Lactic acid bacteria (LAB) isolates. However, some commonly used primers failed to amplify some of my LAB isolates. Can anybody suggest the best primer...

06 May 2018 8,335 16 View

What does the raw variance-covariance ascii data file contain in GROMACS ?

I performed Principal Component Analysis (PCA) using GROMACS 5.1.3 with the following command gmx covar -f filename.xtc -s su2-now.gro -n protein-H.ndx -o eigval.xvg -v eigenvec.trr -ascii...

15 August 2016 8,022 8 View

Why is the combination of nose-hoover thermostat and MTTK barostat not working during NPT equilibriation of a protein in GROMACS ?

I tried to perform NPT equilibriation on a protein with nose-hoover thermostat and MTTK barostat with the following .mdp file ; VARIOUS PREPROCESSING OPTIONStitle = NPT simulation (constant...

11 July 2016 1,759 8 View

How to modulate velocity rescaling in gromacs during heating of a protein ?

I was trying to heat my protein system from 0 K to 300 K using the following temperature coupling tcoupl = v-rescale ; modified Berendsen thermostattc-grps = system ; one coupling grouptau_t = 0.1...

31 May 2016 7,956 16 View

How can I run a MD simulation in gromacs keeping protein heavy atom fixed ?

01 May 2016 6,517 4 View

How to perform SEM analysis using lavaan and R-studio software?

Dear all, I am trying to apply SEM (for the first time) to my graduate research, and I have been learning about SEM and lavaan from online resources and examples, but when it comes to my own...

16 November 2015 4,916 7 View

What are the most relevant MSc proposal topics for Petroleum Production Engineering at the moment?

I am a Petroleum Production Engg MSc student at Robert Gordon University in Aberdeen and I am in the process of selecting a topic for my MSc Engineering Investigation proposal. I would very much...

01 March 2021 7,396 4 View

How to model Supercritical water gasification (SCWG) process in Homer software?

Hi everyone How to model Supercritical water gasification (SCWG) process in Homer software?

25 February 2021 4,075 1 View

How we can measure Kemp % in wool without using of benzene?

Would you please let me to know about the methods for measurement of medullation and Kemp % on animal? My problem is that the wool sampling and sending to laboratory is not possible. So I would...

21 February 2021 7,260 4 View

What are the potential shell well and atomic solvation parameters for the boron atom?

I have a protein-ligand docking project. The ligand contains boron atoms that are not detected by Autodock software. To solve this problem, it is necessary to introduce the boron atom to...

19 February 2021 1,236 1 View

What is the best substrate for exfoliating few layers of hexagonal boron nitride (hBN) using traditional scotch tape method?

Unlike graphene, it is usually very difficult to exfoliate few layers (~10 nm thick) of hBN on SiO2 substrate due to lip-lip interaction at the edges. Au substrates have been proven very useful...

16 February 2021 2,635 2 View

Is secondary metabolite production more at vegetative growth stage or during reproductive stage?

Hi, I am working on the effect of heavy metals on a medicinal plant by pot experimentation. Analyzing the effect of heavy metals on secondary metabolites is one of the parameters. I will be...

11 February 2021 6,683 6 View

Solar energy production forecasting ?

Hello everyone! I am interested in the topic of solar energy production forecasting for a ground-mounted PV system of more than 100MW capacity. Can anyone please suggest what are the main data...

08 February 2021 6,834 5 View

SARS-CoV-2 Spike Protein Western Bands?

Hello, I am western blotting SARS-CoV-2 spike protein from lentiviral using an antibody against S1. To prepare samples, I mixed concentrated lentivirus 1:1 with 2X Laemmli buffer and boiled...

07 February 2021 3,643 4 View

Can I check GFP transfection in 293T cells by flow cytometry?

I would like to troubleshoot the transfection stage of my lentiviral transduction protocol. In short, I have 4 lentiviral plasmids, one of which encodes GFP. I tried transfecting the HEK293T...

24 January 2021 2,341 3 View

What are the different subclasses in intermediate seeds?

I am currently working on an endemic tree species, that produces mature seeds (moisture content 51%) which shows both vivipary (30% of total harvested seeds from a tree) and dormancy (~40%) of...

20 January 2021 2,670 3 View