I need help identifying this contaminate. I think it's a growing organism and I believe it's a fungus but can't be sure. Is it possible that they are crystalline structures precipitating out of solution? I've attached images of my culture at 10x, 20x, and 40x.
My first idea was that it looks like a fungus, but the third picture reminds me of crystal needles. If you want to kow, you could try to stain it, or check if it grows on a common fungal medium like PDA (potato dextrose agar).
I agree that it looks fungal.
Do you include Penicillin-Streptomycin or Antibiotic-Antimycotic in your reagents? Anti-Anti should be more effecting than Penn-Step for fungal control, though both are just bandaids for bad equipment/handling/reagents.
You could probably check to see if it's fungal or bacterial by plating it onto LB-Agar at 37*C for bacteria like e. coli and YPT-Agar at 30*C for fungi like yeast.
It's look like fungl of fly :)
I think you can pick up a little and have a PCR test with fungal 18s primer
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