Can you help me with 5-methylcytosine immunohistochemistry on mouse tissue?

More Mariela Chertoff's questions See All

Call for Papers: Meta-Analysis on Stress and Memory Performance?

Dear Researchers, Greetings from Switzerland! I am reaching out to you as a doctoral student conducting a meta-analysis on the relationship between stress (including test anxiety) and memory...

27 April 2024 9,279 0 View

Can you recommend grants for scientific communication activities?

I'm looking for sources of funding for science communication activities. Do you have grants or calls you can recommend? I am from Latin America and work in the fields of biology, entomology, and...

14 March 2024 2,770 1 View

Can you provide me with Articles about Discourse connectors in writing in EFL?

Need articles related to discourse connectors in EFL writing

18 February 2024 5,523 3 View

Tips for THP-1 differentiated to macrophages immunostaining?

Hi, We're experiencing troubles with THP-1 differentiated to macrophages immunostaining. The nucleus is not uniformly stained with Hoescht, not even the protein we want to stain. Any suggestion on...

19 December 2022 4,160 0 View

THP-1 differentiated to macrophages transfection?

Hi, we[ve tried to transfect THP-1 differentiated to macrophages with Lipo 3000, but the majority of cells died or they are horrible, with clear signs of apoptosis. Any suggestion please? Thanks!

19 December 2022 2,157 1 View

Prices on Spectro flow cytometers?

Hi, I'm looking for prices on ID7000 Sony spectro flow cytometer, BD FACSSymphony A5 SE and Cytek Aurora. Any info? Thanks

23 March 2022 1,702 0 View

Procrustes ANOVA with random effect?

Hi, I'm a beginner using geomorph package, and I wanted to know if it's possible to add a random effect to models to run Procrustes ANOVAs. Is it possible to perform mixed models within the...

15 May 2020 9,998 0 View

What is the time a mycotoxin can be frozen ?

I have cow milk and soft cheese samples frozen. How do I secure the mycotoxin still pure with no alternate concentration? Should I analyze ? I will analyze it by affinity chromatography. Any other...

10 October 2018 8,416 9 View

I am interested in the epigentic regulation of the genetic expression. Which is the best option to measure RNA of blood (whole or white cells)?

Hello I have to measure blood RNA and I would like to ask for advice on whether it is better to use whole blood or eliminate red blood cells. What is the best strategy? How much do red blood cells...

02 September 2018 1,827 4 View

Hello, I would like to know why is necessary a DNA methylase in the GeneArt Site-Directed mutagenesis system ?

I dont understand why is necessary to use a DNA methylase for the amplification step

28 May 2018 6,319 3 View

Do I need specific antibodies to stain for a fusion protein?

I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...

07 August 2024 5,338 2 View

How to determine positive-stained cells in FACS? Use isotype or unstained control?

To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...

06 August 2024 6,728 6 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,710 1 View

Senescence-associated beta galactosidase staining is False Positive in control group?

Hi guys If anyone is currently working on aging cells, you guys would like to give me some advice. I'm testing against biomarker (SA-beta-Gal), I encountered a false positive in the control group...

02 August 2024 6,735 1 View

Does continues partial charging/discharging do any harm to the BESS performance and degradation?

For a grid connected BESS (lets say 100 MW/200 MWh system, LFP battery), if we charge the BESS in such a way that whenever we can access power from the solar that will charge the BESS. Hence it...

01 August 2024 9,903 7 View

How to Post-fix Mice pup's brains?

I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...

31 July 2024 1,123 4 View

Suggestions for the Annexin V Positive Control ?

Hello everyone, I am currently using washed human platelets to stain Annexin V as a procoagulant marker. Additionally, I am staining with PerCP-CD61 to identify platelet cells. So far, I have...

29 July 2024 8,624 1 View

How can I get clearer background for my western blot?

I am running a western on HIV envelope protein. The size is around 50Kda and I am using rat anti-HA for the first stain and a second stain of mouse anti-CA. Primary antibody dilution is 1: 1000...

29 July 2024 6,484 5 View

How many events should be counted for the analysis of apoptosis with nuclear staining with Hoechst 33342 whit microscopy ?

Greeting How many events should be counted for the analysis of apoptosis with nuclear staining with Hoechst 33342 whit microscopy ? Are 100 events count sufficient and three repetitions for...

25 July 2024 7,520 0 View

I am trying to find phonon, and when i run calculations, there is error ?

how to fix this error, from phq_readin : error # 1 DFPT with the Blochl correction is not implemented I am attaching my ph.in file.

23 July 2024 4,649 2 View