Mariela Chertoff

3 Questions 4 Answers 0 Followers

Questions related from Mariela Chertoff

I am interested in the epigentic regulation of the genetic expression. Which is the best option to measure RNA of blood (whole or white cells)?

Hello I have to measure blood RNA and I would like to ask for advice on whether it is better to use whole blood or eliminate red blood cells. What is the best strategy? How much do red blood cells...

03 September 2018 1,806 4 View

How to recover signal on long time postfixed tissue?

Dear all, I have mouse brain tissue perfused with PFA 4% and post fixed in formol 10% for periods between 2 month and 1 year. I cut them on vibratome (30um) and I made inmunohistochemistry and...

23 March 2017 6,453 4 View

Can you help me with 5-methylcytosine immunohistochemistry on mouse tissue?

I am working with 50 µm slices fixed on 4% of PFA. Has anyone experience with 5-metcyt immunohistochemistry in brain tissue? I believe that the protocol might be similar to BrdU staining, but I am...

11 March 2015 7,670 3 View