I have cow milk and soft cheese samples frozen. How do I secure the mycotoxin still pure with no alternate concentration? Should I analyze ? I will analyze it by affinity chromatography. Any other suggestions would be great!
Frozen at which temperature? Changes will occur faster at -20 than at - 80. Which mycotoxin is your target ? Decay will most likely differ between the various mycotoxins.
*· Aflatoxin M1 in Cow’s Milk: Method Validation for Milk Sampled in Northern Italy
Alberto Bellio,† Daniela Manila Bianchi,1,*† Monica Gramaglia, Andrea Loria, Daniele Nucera, Silvia Gallina,1 Marilena Gili, and Lucia Decastelli
Toxins (Basel). 2016 Mar; 8(3): 57. Published online 2016 Feb 26. doi: 10.3390/toxins8030057
Mycotoxins are secondary metabolites of various different fungal species and they differ in chemical structure, biosynthetic origins, and biological effects. Clinicians classify mycotoxins as hepatotoxins, nephrotoxins, neurotoxins, immunotoxins, and so forth, whereas cell biologists classify them as teratogens, mutagens, carcinogens, and allergens. Aflatoxin, for example, is a hepatotoxic, mutagenic, carcinogenic, polyketide-derived Aspergillus mycotoxin . Of the over 300 types of mycotoxins identified so far, aflatoxins (AFs) are the most extensively studied and one of most important classes from a public health perspective. AFs figure among the most serious and well-known naturally occurring toxins in food and feed, with AFB1 being the most toxic and carcinogenic
The choice of which analytical process is most appropriate will take into account the target molecule, chemical features, complex matrix, timing of testing, and required limits of detection/quantification.
Aflatoxin research started in the 1960s. There is a book from 1989 Mycotoxins in Dairy Products H.P van Egmond editor , Publisher Elsevier ; this might be of great help to you
Not all the old information might be easily available on the internet, but I am certain that there will be WHO/FAO documents on this topic as well, so check their websites
At -32 C the micotoxin, especially M1 can be adequately preserved prior to analysis
Dr. Enujiugha
Thank you very much for your response, Do you have any publication or research that allows me to support it?
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